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LC and LC/MS ZORBAX 300Å Extend-C18 ZORBAX 300Å Extend-C18 Hardware Description Size (mm) 696 www.agilent.com/chem/supplies Particle Size (µm) Part No. Analytical 4.6 x 250 5 770995-902 Analytical 4.6 x 150 5 773995-902 Rapid Resolution 4.6 x 150 3.5 763973-902 Rapid Resolution 4.6 x 100 3.5 761973-902 Rapid Resolution 4.6 x 50 3.5 765973-902 Narrow Bore RR* 2.1 x 150 3.5 763750-902 Narrow Bore RR* 2.1 x 100 3.5 761775-902 Narrow Bore RR* 2.1 x 50 3.5 765750-902 Guard Cartridge, 4/pk 4.6 x 12.5 5 820950-932 Guard Cartridge, 4/pk 2.1 x 12.5 5 821125-932 Guard Hardware Kit 820888-901 Capillary Glass-lined Columns Capillary RR* 0.3 x 150 3.5 5065-4464 Capillary RR* 0.3 x 100 3.5 5065-4465 Capillary RR* 0.3 x 75 3.5 5065-4466 Capillary RR* 0.3 x 50 3.5 5065-4467 *RR: Rapid Resolution 3.5 µm
ZORBAX GF-250 and GF-450 Gel Filtration Columns ZORBAX GF-250 and GF-450 Gel Filtration Columns • High efficiency and reproducibility with short analysis time • Hydrophilic diol bonded phase for good protein recovery • Compatible with organic modifiers and denaturants • Wide usable pH range (pH 3-8) Agilent ZORBAX GF-250 and GF-450 size exclusion (gel filtration) columns are ideal for the size separations of proteins and other biomolecules. The separation range is 4,000-900,000 for globular proteins when using GF-250 and GF-450 columns in series. The GF-250/GF-450 size exclusion columns have a hydrophilic diol bonded phase for high recovery of proteins (typically >90%) and a unique zirconia modification of the silica to extend the pH range from 3-8. The GF-250 and GF-450 columns are packed with precisely sized porous silica microspheres with narrow pore size and particle size distributions. The result is a highly efficient, rugged and reproducible size exclusion column for separations of proteins with flow rates of up to 3 mL/min. These columns are compatible with organic modifiers (
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TABLE OF CONTENTS Featured Products
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7683B Automatic Liquid Sampler Deli
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Number of pesticide hits Number of
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Agilent 1200 Series LC Systems and
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1200 Series Purification Systems De
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Agilent 6300 Series Ion Trap LC/MS
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Make the Change to ICP-MS with Agil
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BioCel Automation Systems Through i
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Stratagene Real-time PCR Solutions
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MS Certified Liners www.agilent.com
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Focus on what you do best, and leav
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Agilent Offices Worldwide Australia
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Brazil Hexis Científica Jundiaí,
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Japan Yokogawa & Co., Ltd. 7-336, K
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Thailand Worldco Co. Ltd 63/3 Arun-
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General Chromatography Supplies Con
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Agilent Vial Identification Chart a
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Screw top vials with fixed inserts
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Preassembled vial pack Pre-Assemble
Page 39 and 40:
Magnetic crimp caps for CTC, 5188-5
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Snap caps with septa Snap Top Caps
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Polypropylene Crimp/Snap Top Vials
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Micro-V Vials • Wide opening, 1.5
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8-425 screw top vials 8-425 Screw T
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4 mL screw top vials 4 mL Screw Top
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Certified headspace crimp top vials
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General Purpose Sample and Storage
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Manual crimper and decapper Micro v
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Syringes With a broad selection of
Page 59 and 60:
Straight Needle, 23 and 26s Gauge A
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10 µl fixed needle 25-500 µL fixe
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On-column syringe Push pull Push bu
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CTC Analytics PAL Autosampler Suppl
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Fixed needle, G6500-80109 CombiPAL
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X-type fixed needle, G4200-80118 Re
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Sample tray, G6500-80101 Well plate
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Carrier Gas Purification Gas supply
Page 75 and 76:
Gas Purifier Selection Guide In Lin
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a b (Figures a and b. The “plus
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Triple filter cartridge, 5182-9705
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Gas Traps www.agilent.com/chem/supp
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Molecular Sieve 5A and Indicating D
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Moisture S-trap Big moisture trap M
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Big oxygen trap Big hydrocarbon tra
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Capillary Grade Hydrocarbon Traps (
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Hydrocarbon Moisture trap Hydrocarb
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Split vent trap and cartridges, RDT
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ADM 1000 ADM 2000 ADM 1000 www.agil
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Low Gas Alarm System, 2.0 and 2.5 i
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www.agilent.com/chem/supplies Regul
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Fittings kit, 5180-4161 GC Installa
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Brass adapter, 0100-0420 Stainless
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Reducing union, 0100-0241 Reducing
Page 107 and 108:
Hex Keys, 5064-8211 Screwdrivers De
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Capillary and Megabore ferrule tool
Page 111 and 112:
FID cleaning kit, 9301-0985 GC Budd
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Ink cartridge, 5181-1220 Integrator
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HPIB and GPIB Cables Integrators, I
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Sample Preparation Improve the qual
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Move more quickly toward the method
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R 1 Sulfonic acid-modified divinyl
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O Si O Si (CH2) 17CH3 Si O Si(CH 3)
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Structure of ethyl (C2) silane, cov
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Si O Si O Si OH Structure of diol s
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Additional Agilent SampliQ SPE Phas
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SampliQ Carbon SampliQ Carbon (grap
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Agilent SampliQ Bulk Sorbents www.a
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20-Port Vacuum Manifold Assembly, 5
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Vacuum Manifolds for 96 Well Plates
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Why Filter Your Samples for HPLC?
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Membrane Econofilters, 5185-5830 Re
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Tips & Tools For more information,
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GC and GC/MS For over 40 years, Agi
Page 147 and 148:
GC and GC/MS Maintenance Schedule w
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BTO septa, 5183-4757 Summary of Pre
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Septa Troubleshooting General Purpo
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MS certified split liner, 5188-6576
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Agilent splitless liners Single tap
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Certified gold inlet seal, 5188-536
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Ferrule Selection Recommendations w
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Universal column nut, 5181-8830 Col
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Glass press-fit connections Quartz
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Capillary Flow Technology Supplies
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A B C Valve Supplies Description Pa
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Markes Thermal Desorption Markes Th
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Bar code reader labels Automatic Li
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G1888A Headspace unit G1888A Networ
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Flip Top Inlet Sealing System insta
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Split/Splitless Inlet assembly Spli
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1a 5 6 1b 2 3 1c 1d Cool On-Column
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Tips & Tools For new PTV technology
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Flame Ionization Detector (FID) FID
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FID cleaning kit, 9301-0985 Flame I
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Detector fitting Front ferrule Back
Page 189 and 190:
www.agilent.com/chem/supplies Flame
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www.agilent.com/chem/supplies Nitro
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13 14 17 18 1 3 4 5 6 15 16 8 7 Nit
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Sulfur Chemiluminescence Detector (
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Cool On-Column Inlets for Manual In
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7 5890 FID 2 3 4 5 8 9 6 Flame Ioni
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ECD make up gas adapter ECD packed
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GC Standards GC Qualitative Standar
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GC/MS Systems • Less downtime for
Page 207 and 208:
MS interface column nut, 05988-2006
Page 209 and 210:
Electron Impact (EI) Ion Source Ion
Page 211 and 212:
Tips & Tools Visual appearance is n
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EI high temperature filament, G2590
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Foreline Pump Vacuum Systems and Pu
Page 217 and 218:
MS Test and Performance Samples MS
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Always Quality. Always Innovative.
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Tips & Tools See where quality begi
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Agilent's Über One Test Probe Mixt
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High Efficiency Capillary GC Column
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Spearmint Oil Carrier: A: Helium 25
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Structure of Dimethylpolysiloxane D
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CH 3 CH 3 CH 3 O Si Si O Si CH3 CH3
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CH 3 O Si CH 3 Structure of Dipheny
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CH 3 DB-35ms Chromatograms Environm
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DuraGuard www.agilent.com/chem/supp
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Structure of Dimethylpolysiloxane D
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DB-1 www.agilent.com/chem/supplies
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HP-1 0.32 15 0.25 -60 to 325/350 19
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DB-5 www.agilent.com/chem/supplies
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HP-5 www.agilent.com/chem/supplies
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CH 3 O Si O Si Structure of Dipheny
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O CH 3 C 6H 5 Si O Si CH 3 50% C 6H
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O Si Structure of trifluoropropylme
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DB-1301 • (6%-Cyanopropyl-phenyl)
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-HO H H C C O H H Structure of Poly
Page 259 and 260:
DB-WAX and DB-WaxFF www.agilent.com
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HP-INNOWax Chromatograms Food, Flav
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HP-FFAP Chromatograms Food, Flavors
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Chiral Columns Special Application
Page 267 and 268:
Cyclodex-B Chromatograms Food, Flav
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DB-5ht Chromatograms Food, Flavors
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DB-EVDX Chromatograms Life Sciences
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DB-608 Chromatograms Environmental
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DB-HT SimDis Chromatograms Petroleu
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DB-Dioxin Chromatograms Environment
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DB-VRX Chromatograms Environmental
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HP-VOC • Selectivity engineered f
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PLOT Columns www.agilent.com/chem/s
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GS-Alumina KCl Chromatograms Petrol
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GS-GasPro Chromatograms Environment
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HP-PLOT Q Chromatograms Environment
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Metal Columns DB-ProSteel • Excel
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CAM Chromatograms Industrial Chemic
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Custom GC Column Ordering Even thou
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DB-1, 5 in. cage ID (mm) Length (m)
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DB-17, 5 in. cage ID (mm) Length (m
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DB-1701P, 5 in. cage ID (mm) Length
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GS-Q, 5 in. cage ID (mm) Length (m)
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HP-20M, 5 in. cage ID (mm) Length (
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HP-PLOT Molesieve, 5 in. cage www.a
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Agilent LTM System CAM LTM Column M
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0.20 12 0.33 -60 to 325/350 128-101
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DB-1ms LTM Column Modules ID (mm) L
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DB-17ms LTM Column Modules ID (mm)
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DB-23 LTM Column Modules ID (mm) Le
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DB-5 LTM Column Modules ID (mm) Len
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DB-5.625 LTM Column Modules www.agi
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DB-WAX LTM Column Modules www.agile
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GS-Q LTM Column Modules ID (mm) Len
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HP-5 LTM Column Modules www.agilent
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HP-PLOT Al2O3 "M" LTM Column Module
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HP-PLOT Q LTM Column Modules www.ag
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Fused Silica Tubing Deactivated Tub
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GC Column Application and Method Gu
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GC Columns Stationary Phase Applica
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GC Columns Stationary Phase Applica
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Environmental/EPA Methods Analyte T
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Environmental/EPA Methods EPA Metho
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ASTM Methods Method Designation Met
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Baseline Instability or Disturbance
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Loss of Resolution GC Column Troubl
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www.agilent.com/chem/supplies Solve
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Alphabetical Order By Analyte (Cont
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Environmental Applications, Hydroca
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Environmental Applications, Pestici
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Organochlorine Pesticides I EPA Met
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Chlorinated Pesticides, EPA Method
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Organochlorine Pesticides III Colum
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Nitrogen/Phosphorus Containing Pest
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Herbicides II Column: DB-210 122-02
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Environmental Applications, Semivol
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Semivolatile Compounds, EPA Method
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PBDEs by ECD Column: DB-XLB 15 m x
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PCBs by EPA Method 8082 Column: DB-
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Organotin Compounds II Column: HP-5
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EPA Method 8061 (Phthalate Esters)
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Phenols Column: DB-5ms 122-5532 30
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Environmental Applications, Volatil
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Extended Analyte List for EPA Metho
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EPA Method 551 Column: DB-1 122-103
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EPA Air Analysis Method TO-15 (1 pp
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C1 and C2 Halocarbons (Freons) Colu
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Foods, Flavors and Fragrance Applic
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Fragrance Reference Standard II Col
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Flavor Mixture Column: ULTRA 2 1909
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Peppermint Oil Column: DB-WAX 122-7
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Rosemary Oil Column: Cyclosil-b 112
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Strawberry Syrup Column: HP-INNOWax
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Organic Acids Column: DB-FFAP 122-3
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Polyunsaturated Fatty Acid Methyl E
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FAME Standard II Column: DB-225 127
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Butter Triglycerides I Column: DB-5
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Alcohols II Column: DB-WAXetr 123-7
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Free Organic Acids/C4-C5 Isomers Co
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Amines and Nitriles Column: DB-5ms
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Aldehydes and Ketones Column: DB-1
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PFBHA Derivative Column: DB-1 123-1
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Impurities in Styrene Column: DB-WA
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Esters I Column: DB-1 125-1034 30 m
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Glycols I Column: DB-WAX 124-7032 3
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Ethylene Glycol Mixture Column: ULT
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Halogenated Hydrocarbons II Column:
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Solvents II Column: DB-WAXetr 123-7
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Common Industrial Solvents Column:
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Acrylate Impurities II Column: DB-1
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Phenols I Column: HP-5ms 19091S-433
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Ethylene Oxide Column: DB-WAX 122-7
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Life Science Applications Benzodiaz
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Drug Screen Column: DB-1ms 122-0132
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Anticonvulsants Column: DB-1 125-10
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Barbiturates Column: DB-35ms 122-38
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Hallucinogens Column: DB-17ms 122-4
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Over-the-Counter Pain Killers - TMS
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Blood Alcohols I (Static Headspace/
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Blood Pollutants II Column: DB-ALC1
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Residual Solvents Column: DB-624 12
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Selected Oxygenates Column: GS-OxyP
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Natural Gas Column: HP-PLOT Al2O3 S
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Propylene Column: GS-Alumina 115-35
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Extended Hydrocarbon Analysis I Col
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Refinery Gas I Column: HP-PLOT Q 19
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Sulfur Gas Analysis in Light Hydroc
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Sulfur Compounds in Propylene (1 pp
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Aromatics Analysis - Ethylbenzene I
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Denatured Fuel Ethanol-ASTM D5501 C
Page 483 and 484:
Simulated Distillation Column: DB-2
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n-Paraffin Standard Column: DB-HT S
Page 487 and 488:
LC and LC/MS Run samples up to 20x
Page 489 and 490:
Universal Connection Capillaries
Page 491 and 492:
Plastic tubing cutter, 8710-1930 PE
Page 493 and 494:
Stainless steel fittings, 5062-2418
Page 495 and 496:
Semi prep filter, 5064-8273 High pr
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Glass solvent filter degasser, 3150
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www.agilent.com/chem/supplies Manua
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Stainless steel sample loops www.ag
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Syringes for manual injection valve
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1100/1200 Series LC Maintenance Sch
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Mounting tool, 0100-1710 Capillary
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Pumps www.agilent.com/chem/supplies
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Outlet ball valve, G1311-60012 Acti
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Pump start up kit, G1311-68710 Solv
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Prep filter, 5065-4500 Prep bottle,
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1100/1200 Series LC System Needles
Page 519 and 520:
Sample loop, 01078-87302 www.agilen
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G1367A well plate autosampler G1387
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G2255-68700 Vial plate, 5022-6539 W
Page 525 and 526:
G2260A 1100/1200 Series Preparative
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1100/1200 Series Fraction Collector
Page 529 and 530:
1200 Series LC Solution Kits Biocom
Page 531 and 532:
1100/1200 Series LC System Connecti
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1200 Rapid Resolution Connection Ca
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Standard flow cell Standard flow ce
Page 537 and 538:
Micro flow cell High pressure flow
Page 539 and 540:
8 Deuterium longlife lamp, 2140-081
Page 541 and 542:
Diode Array Detector (DAD)/Multiple
Page 543 and 544:
Diode Array Detector (DAD)/Multiple
Page 545 and 546:
Stainless steel front ferrules, 518
Page 547 and 548:
Piston seals, 5063-6589 PTFE frits,
Page 549 and 550:
Deuterium lamp, G1314-60100 Variabl
Page 551 and 552:
6100 Series Single Quadropole LC/MS
Page 553 and 554:
6200 Series TOF LC/MS Supplies www.
Page 555 and 556:
6300 Series Ion Trap LC/MS Supplies
Page 557 and 558:
6400 Series Triple Quadropole LC/MS
Page 559 and 560:
6500 Series Accurate-Mass Q-TOF LC/
Page 561 and 562:
Quiet Cover Quiet Cover Agilent has
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Column and Mobile Phase Guidelines:
Page 565 and 566:
pH and Mobile Phase The choice of m
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Analyte charge Silica surface charg
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Method development at mid pH (4-9)
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USP Designations The US Pharmacopei
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USP Designations USP Packing Materi
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USP Designations USP Packing Materi
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Cartridge/Guard Cartridge Systems C
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This icon identifies stand-alone gu
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This icon identifies preparative gu
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HPLC Column Protection Column Prote
Page 585 and 586:
ZORBAX Rx-SIL uniform sub particles
Page 587 and 588:
ZORBAX Reversed-Phase Columns The f
Page 589 and 590:
Poroshell 120 • High efficiency a
Page 591 and 592:
ZORBAX Eclipse Plus: Best Peak Shap
Page 593 and 594:
ZORBAX Eclipse Plus Hardware Descri
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ZORBAX Eclipse PAH Hardware Descrip
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Good Peak Shape Over a Wide pH Rang
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Selectivity Changes for Basic Compo
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ZORBAX Eclipse XDB Hardware Descrip
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ZORBAX Eclipse XDB Hardware Descrip
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StableBond SB-C18 Shows Excellent S
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Five Different Bonded Phases Provid
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ZORBAX 80Å StableBond Hardware Des
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ZORBAX 80Å StableBond www.agilent.
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Improved Peak Shape of Basic Compou
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ZORBAX Bonus-RP Provides Unique Sel
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O O Si Si Silica Support C18 C18 No
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Extend-C18 Provides Good Peak Shape
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ZORBAX 80Å Extend-C18 Hardware Des
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ZORBAX Rx Hardware Description Size
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LiChrospher Reversed-Phase Cartridg
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Asahipak Reversed-Phase Columns Asa
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ZORBAX Normal Phase Columns For nor
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Normal Phase Columns Based on ZORBA
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LiChrospher Normal-Phase Cartridge
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Cough-Cold Remedies - ZORBAX 300SCX
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Poroshell 120 • High efficiency a
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ZORBAX Rapid Resolution High Throug
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Rapid Resolution HT Provides Double
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Long Lifetime of RRHT Columns at El
Page 645 and 646: ZORBAX Rapid Resolution High Throug
Page 647 and 648: Rapid Resolution Columns Reduce Ana
Page 649 and 650: ZORBAX Rapid Resolution 3.5 µm Ecl
Page 651 and 652: ZORBAX Solvent Saver • Provide 60
Page 653 and 654: ZORBAX Eclipse Plus Description Siz
Page 655 and 656: Separation of a Tryptic Digest on Z
Page 657 and 658: ZORBAX PrepHT • Easy scale-up fro
Page 659 and 660: ZORBAX PrepHT columns are designed
Page 661 and 662: ZORBAX PrepHT Eclipse XDB (require
Page 663 and 664: Agilent Prep LC Columns • High lo
Page 665 and 666: Agilent Prep LC Columns www.agilent
Page 667 and 668: Separation of Ethiazide (diuretic d
Page 669 and 670: GPC-SEC start-up kit GPC-SEC Start-
Page 671 and 672: Organic Standard Chromatograms Aque
Page 673 and 674: PLgel Columns Agilent Technologies
Page 675 and 676: Polystyrene: Calibration Curves for
Page 677 and 678: Separation of Simple-Sugar and Suga
Page 679 and 680: High Resolution of 24 Amino Acids U
Page 681 and 682: ZORBAX Method Development Kits ZORB
Page 683 and 684: ZORBAX Method Validation Kits ZORBA
Page 685 and 686: Porous Shell SOLID CORE 5 µm Poros
Page 687 and 688: MicroBore Poroshell 300 Columns Pro
Page 689 and 690: Short-Chain ZORBAX 300SB-C3 is Stab
Page 691 and 692: ZORBAX 300Å StableBond Hardware De
Page 693 and 694: Novel Bidentate C18-C18 Bonding for
Page 695: LC/MS Analysis of Angiotensin on Ex
Page 699 and 700: ZORBAX GF-250 Shows Extended Column
Page 701 and 702: ZORBAX Bio-SCX Series II Provides M
Page 703 and 704: High Sensitivity with Capillary Col
Page 705 and 706: Proteins in a Complex Sample by 2-D
Page 707 and 708: Multiple Affinity Removal System -
Page 709 and 710: Column Specifications Multiple Affi
Page 711 and 712: Multiple Affinity Removal System -
Page 713 and 714: Tips & Tools The Spin Cartridge Rea
Page 715 and 716: Luer-Lok syringe, 5188-5250 Luer-Lo
Page 717 and 718: Protein In-gel Tryptic Digestion Ki
Page 719 and 720: Peptide Cleanup Pipette Tips Peptid
Page 721 and 722: HPLC Troubleshooting Symptom Type P
Page 723 and 724: LC/MS Troubleshooting Symptom Type
Page 725 and 726: Analgesics: High-Speed PrepHT Isocr
Page 727 and 728: Cough Formula Mixture: Fast and Eff
Page 729 and 730: Use ZORBAX Extend-C18 for Alternate
Page 731 and 732: Peptides: Effect of TFA Concentrati
Page 733 and 734: Peptide Map - Electronic Flow Contr
Page 735 and 736: Peptide RP-HPLC/ESI-MS Using NH4OH
Page 737 and 738: Catecholamines/Biogenic Amines: Rap
Page 739 and 740: Proteins: Effect of Bonded Phase Co
Page 741 and 742: BSA Tryptic Digest on RRHT Column:
Page 743 and 744: Polymethacrylate: GPC Separation Co
Page 745 and 746: Triton X-114: Decreasing Run-time b
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Organic Acids Separated on ZORBAX S
Page 749 and 750:
Anilines, Separation of Substituted
Page 751 and 752:
Explosives from Soil Extract Column
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Herbicides: Use of Short, Small Par
Page 755 and 756:
Triazine Pesticides on Bonus-RP and
Page 757 and 758:
Plant Hormones: Rapid Gradient Elut
Page 759 and 760:
Aromatic Flavorings Column: Eclipse
Page 761 and 762:
Carbohydrates in Colas Column: ZORB
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Flavoring Agents Column: ZORBAX SB-
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Parabens: High Speed Separation Col
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Water-Soluble Vitamins Column: ZORB
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Fast Vitamin E Analysis on Rapid Re
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Xanthines: Higher Resolution, Same
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Anesthetics, Local: Bonded Phase Se
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Antihistamines Column: ZORBAX Rx-C8
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Chiral Separation of Fluoxetine Ena
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Chiral Separation of Tolperison Ena
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Metronidazole: Updating USP Methods
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Opiate Drugs, Basic Compounds Showi
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Steroids Column A: Eclipse XDB-Phen
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Tricyclic Antidepressants, Screenin
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Dexamethasone, USP Method: Rapid An
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Sulfonamides - Fast Analysis with R
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CE and CE/MS Achieve the sensitivit
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Tips & Tools Agilent offers a varie
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µPAGE Basic Kits Includes 3 µPAGE
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Extended Light Path (Bubble Cell) B
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www.agilent.com/chem/supplies CE an
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www.agilent.com/chem/supplies CE an
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Alignment Interfaces Capillary cass
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CE/MS Accessories www.agilent.com/c
Page 809 and 810:
CE Standards & Reagents Premade buf
Page 811 and 812:
CE System Start-up and Test Kits Ch
Page 813 and 814:
Accessories www.agilent.com/chem/su
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ICP-MS The power you need for ultra
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Gas Line Supplies www.agilent.com/c
Page 819 and 820:
Peristaltic Pump Tubing and Supplie
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Concentric Nebulizer, quartz Use wi
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Quartz spray chamber, G1820-65337 S
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Interface Cone Guide for Agilent IC
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Electron multiplier, 5184-1983 Ion
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Integrated Autosampler Supplies Des
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ISIS Supplies www.agilent.com/chem/
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Miscellaneous Supplies Description
Page 835 and 836:
Single Element Standards Descriptio
Page 837 and 838:
UV-Vis Keeping your system operatin
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Semi-Micro Cell with PTFE Lid Semi-
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Round Aperture Flow Cell Rectangula
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Deuterium lamp assembly, 2140-0605
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UV-Vis Standards & Reagents Our che
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0905-1175..........................
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123-0131LTM........................
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125-5032E..........................
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19091N-133.........................
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200-0185...........................
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5064-8286..........................
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5183-4428..........................
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5982-4411..........................
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840300-908.........................
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959746-906.........................
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G1530-61580........................
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G3270-80026........................
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1,4-Butanediol ....................
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Dicrotophos .......................
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1,2,3,4,6,7,8-Hepta chloro dibenzof
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Octadecane ........................
Page 879 and 880:
Triethylenetetramine (4 isomers) ..
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Caps 8-425 Screw Top No Septa......
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GC Installation Kits Basic Installa
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Moisture Traps Cont. Silica Gel, Gr
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Vials Cont. 2 mL Glass ............
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Easy Ways To Order* • For Interna
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Agilent Technologies Order Form Agi
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