TABLE OF CONTENTS - BGB Analytik AG

ZORBAX PrepHT columns are designed for rapid scale-up from analytical to preparative scale
without losing resolution. For complex separations for larger columns (21.2 mm ID and
higher), 150 mm length and higher), Agilent has carefully chosen the 7 µm particle size
to achieve a balance between high efficiency and high loadability.
Scale-Up to PrepHT
Abundance (mAU)
3500
3000
2500
2000
1500
1000
500
0
Column 1
0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0
Time (min)
www.agilent.com/chem/supplies
1
2
Column 2
3
Column 3
Column 4
ZORBAX PrepHT
Scale-Up from Analytical to Prep ZORBAX SB-C18 Columns
Using the Same Pump
Flow
Column Size
(mL/min) Injection (µL) Detector Cell Part No.
Column 1 50 x 150 mm 100 2200 0.3 mm quartz Custom Column
Column 2 21.2 x 150 mm 18 400 0.3 mm quartz 877150-102
Column 3 9.4 x 150 mm 3.5 80 0.3 mm quartz 883975-202
Column 4 4.6 x 150 mm 0.85 2.0 3 mm SST 883975-902
Using the same 1100 pump, a scale-up from 4.6 mm to 50 mm ID was possible without any loss of resolution. This increase
throughput by reducing the time required for redeveloping and adjusting the method.
1. Theobromine
2. Theophylline
3. Caffeine
LCPHT_ScaleUp
LC and LC/MS
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