TABLE OF CONTENTS - BGB Analytik AG

Residual Solvents
Column: DB-624
123-1364
60 m x 0.32 mm, 1.80 µm
Carrier: Helium, 35-40 cm/sec, set to yield same
RT for Hexane on all columns.
Oven: 40°C Isothermal
Sampler: Ambient Headspace
Injection: Split ratio 1:8
Detector: FID, 240°C
Sample: 4 µL
The three chromatograms to the right show how widely
different the two other manufacturer’s columns are
compared to the DB-624, the original USP G-43
stationary phase column recommended for the analysis
of these common organic volatile impurities in
pharmaceutical samples.
Top Chromatogram: DB-624
Middle Chromatogram: Manufacturer A's "-624"
Bottom Chromatogram: Manufacturer B's "-624"
www.agilent.com/chem/supplies
Life Science Applications
2 4 6 8 10
Time (min)
Peak Numbers Critical Pair DB-624
% Resolution*
Manufacturer A's 624 Manufacturer B's 624
2,3 ethanol and diethyl ether 100% 0% 0%
5,6 ethyl acetate and 2-butanone 95% 38% 0%
7,8 2-butanol and chloroform 60% 0% 60%
9,10 benzene and isooctane 100% 0% 0%
*Resolution calculated as follows: %R = 100% x valley height/average of peak height 1 + peak height 2
Tips & Tools
pA FID1 A1 100
80
60
40
20
pA FID1 A1 100
80
60
40
20
pA FID1 A1 100
80
60
40
20
1
1
2 4 6 8 10
1
2
2
2
3
3
3
4
2 4 6 8 10
4
4
1. Methanol
2. Ethanol
3. Diethyl ether
4. Hexane
5. Ethyl Acetate
6. 2-Butanone
7. 2-Butanol
8. Chloroform
9. Benzene
10. Isooctane
5 6
7 8
5 6
5, 6
7 8
For the latest residual solvent application for USP 467, request publication number 5989-8085EN.
7, 8
9
9
9 10
10
10
GCLS029
GC and GC/MS
463