TABLE OF CONTENTS - BGB Analytik AG

Selectivity Changes for Basic Compounds
with Eclipse XDB and StableBond
Column A: Eclipse XDB-C8
966967-906
4.6 x 75 mm, 3.5 µm
Column B: ZORBAX Rx/SB-C8
866953-906
4.6 x 75 mm, 3.5 µm
Mobile Phase: 70% 25 mM
NaH2PO4, pH 3.0
30% Methanol
Flow Rate: 1.0 mL/min
Temperature: 35°C
A 2
B
3
0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5
Time (min)
A
1
0 2 4 6 8
B
1
0 2 4 6 8
C
1
3
2
2
0 2 4 6 8 10 12 14 16
Time (min)
www.agilent.com/chem/supplies
1
1. Barbital
2. Sulfamethoxazole
3. Caffeine
0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5
Time (min)
Eclipse XDB and StableBond columns are based on the same silica but have different bonding and endcapping. Therefore, they can have very different selectivity for the
same sample under the same conditions, as this example shows.
Optimize Separations with Eclipse XDB Selectivity
Options: Analysis of Sunscreens
Column A: Eclipse XDB-Phenyl
963967-912
4.6 x 150 mm, 3.5 µm
Column B: Eclipse XDB-C8
963967-906
4.6 x 150 mm, 3.5 µm
Column C: Eclipse XDB-C18
963967-902
4.6 x 150 mm, 3.5 µm
Mobile Phase: 15% H20: 85% MeOH
Flow Rate: 1.0 mL/min
Temperature: 35°C
Sample: Sunscreens
3
ZORBAX Eclipse XDB
1. Oxybenzone
2. Padimate-O
3. Ethylhexylsalicylate
10 12 14 16
10 12 14 16
This separation of sunscreens on all three Eclipse XDB bonded phases – C18, C8 and Phenyl – shows that different bonded phases can be used to optimize a separation.
While all three bonded phases provide an adequate separation, the Eclipse XDB-Phenyl provides a different peak elution order and a much shorter overall analysis time. All
three bonded phases also provide excellent peak shape with no mobile phase additives.
2
1
2
3
3
LCEC007
LCEC008
LC and LC/MS
599