TABLE OF CONTENTS - BGB Analytik AG

Separation of a Tryptic Digest
on ZORBAX MicroBore 300SB-C18
Column: ZORBAX 300SB-C18
863630-902
1.0 x 150 mm, 3.5 µm
Mobile Phase: Gradient: 2-60% B in 60 Min.
A: 0.1% TFA
B: 0.075% TFA/80% ACN
Flow Rate: 50 µL/min
Temperature: 50°C
Detector: 215 nm
Sample: 2 µL
Tryptic Digest of rhGH
This example of a tryptic digest separated on a MicroBore column
demonstrates the high sensitivity and resolution possible with
1.0 mm ID columns.
ZORBAX MicroBore (1.0 mm ID)
• High sensitivity for small sample sizes
• Compatible with LC/MS interfaces
• Wide variety of bonded phases
Agilent ZORBAX MicroBore (1.0 mm ID) columns are often a good choice when sample sizes
are limited. They can improve detection limits 5 times over 2.1 mm ID columns when the same
sample mass is used. This increase in sensitivity can be critical. MicroBore columns use low
flow rates (typically ~ 50 µL/min). Therefore, these columns are ideal for use with detectors
requiring low flow rates such as some mass spectrometers and with capillary LC systems.
MicoBore columns perform optimally with HPLC systems purchased or modified for microbore
use. A wide variety of bonded phases are available for use up to 400 bar including StableBond
SB-C18, SB-C8, 300SB-C18; Eclipse XDB-C18 and XDB-C8; Bonus RP, Extend C-18; and
Poroshell columns. Guard columns are also now available with an adjustable tube stop depth
to provide a perfect zero dead volume connection every time.
0 5
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ZORBAX MicroBore (1.0 mm ID)
10 15 20 25 30 35 40 45 50 55
Time (min)
LCMB001
LC and LC/MS
655