TABLE OF CONTENTS - BGB Analytik AG

Anilines, Separation of Substituted Anilines at pH 2.5
Column: ZORBAX SB-C18
883975-902
4.6 x 150 mm, 5 µm
Mobile Phase: 42% methanol/58% 25 mM phosphate buffer
Flow Rate: 1.0 mL/min
Temperature: 22°C
Detector: UV 254 nm
Sample: Anilines
Anilines, Substituted: Rapid Separation
Column: ZORBAX Rx/SB-C8
866953-906
4.6 x 75 mm, 3.5 µm
Mobile Phase: 20% ACN/80% 25 mM phosphate buffer, pH 2.5
Flow Rate: 3.0 mL/min
Temperature: 60°C
Detector: UV 254 nm
Sample: Anilines
Anions With Agilent HPLC Column
and Mobile Phase Additive (Indirect UV)
Column: Asahipak ODP-50
79923OP-564
4.0 x 125 mm, 5 µm
Mobile Phase: Water/acetonitrile, 86/14% + modifier
(P/N: 5062-2480)
adjusted to pH 8.6 with carbonate
free NaOH
Flow Rate: 1.5 mL/min
Temperature: 40°C
Detector: UV 266 nm
Sample: Anions
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1
2
3
pH 2.5
Environmental Applications
0 1 2 3 4 5 6 7 8 9
Time (min)
0
mAU
60
50
40
30
20
10
0
1
2
0.5
F-
CI-
3
NO2-
4
1.0
Br-
5
4
N = 9000
As = 1.02
6
5
6
1. p-Anisidine
2. m-Toluidine
3. 3-Amino-benzonitrile
4. p-Chloroaniline
5. m-Chloroaniline
6. o-Chloroaniline
LCEN004
1.5 2.0
Time (min)
2.5 3.0 3.5 4.0
NO 3- H2PO4 2-
1. p-Anisidine
2. m-Toluidine
3. 3-Amino-benzonitrile
4. p-Chloroaniline
5. m-Chloroaniline
6. o-Chloroaniline
SO 4 2-
LCEN005
2 4 6 8 10 12
Time (min)
LCEN006
LC and LC/MS
749