TABLE OF CONTENTS - BGB Analytik AG

LC and LC/MS
ZORBAX Normal Phase Columns
Column Specifications
High Resolution Normal Phase Separation
of Octylphenoxy Ethanol Surfactant on ZORBAX CN
Column: ZORBAX CN
880952-705
4.6 x 250 mm, 5 µm
Mobile Phase: Primary: Heptane
Secondary: 2-Methoxyethanol/Isopropanol
(50/50)
Flow Rate: 2 mL/min
Gradient: 2-20% Secondary in 10 min., Linear Hold at 20%
Temperature: 50°C
Detector: 278 nm
Sample: Octylphenoxy (polyethylene oxy)
Ethanol Surfactant (n= 10)
0 5
630 www.agilent.com/chem/supplies
Bonded Phase Pore Size
Surface
Area pH Range Endcapped Carbon Load
ZORBAX Rx-SIL 80Å 180 m2/g 0-8.0 No
ZORBAX Eclipse XDB-CN 80Å 180 m2/g 2.0-8.0 Yes 4.3%
ZORBAX SIL 70Å 300 m2/g 0-8.0 No
ZORBAX CN 70Å 300 m2/g 2.0-7.0 Yes 7%
ZORBAX NH2 70Å 300 m2/g 2.0-7.0 Yes 4%
10 15
Time (min)
20
LCNP001