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HA008<br />

Effectiveness of differents methods for cleaning arch wire<br />

Araujo MTS, Canongia ACP, Lima CJ, Figueiredo LM, Puetter UT*,<br />

Sant´Anna EF<br />

Odontopediatria e Ortodontia - UNIVERSIDADE FEDERAL DO RIO DE JANEIRO.<br />

E-mail: monicatirre@uol.com.br<br />

Cleaning orthodontic archwires before reinsert in patients is necessary to reduce bacterial and<br />

fungus biofilm. The aim of this study was to evaluate the best method of removing bacterial<br />

and fungus biofilm. Methods: rectangular NiTi and SS wires segments from 3M and Morelli<br />

and TMA wires from Morelli were separately divided into five groups. The wire segments were<br />

contaminated with strains of S. mutans and C. albicas, with the exception of 4 segments of<br />

each group that served as control. The groups were tested using six biofilm removal techniques:<br />

friction of cotton roll with sodium hypochlorite 1% (RH); friction of cotton roll with 2%<br />

chlorhexidine gluconate (RC); friction of cotton roll with 70% alcohol (RA); immersion in<br />

enzymatic detergent (ID); friction of steel wool (SW) and friction of cotton roll without<br />

chemical agents (R0). The wires were then placed in broth separately, and after an incubation<br />

period, the optical density (OD) was measured to observe whether there was microbial growth.<br />

The wire segments of each group were scanned with Electron Microscope (SEM) for qualitative<br />

and quantitative analysis of treatment response. The data were submitted to one-way ANOVA<br />

test and Tukey post-test. Results: 2% chlorhexidine and 1% sodium hypochlorite removed the<br />

microorganisms completely while the other agents left a high microbial concentration.<br />

HA009 Prospective longitudinal study of the dentoskeletal changes from the<br />

use of the twin-force in the treatment of Class II malocclusion<br />

Guimaraes‐Junior CH*, Henriques JFC, Janson G, Giampietro‐Brandão A,<br />

Henriques RP, Almeida MR, Vedovello‐Filho M<br />

Ortodontia - FACULDADES DA FUNDAÇÃO HERMÍNIO OMETTO.<br />

E-mail: ibepo@terra.com.br<br />

The objective of this study was to evaluate the dentoskeletal changes resulting from the<br />

treatment of Class II malocclusion with a fixed functional appliance, associated with fixed<br />

orthodontic appliance, by means of cephalometric analysis of the 86 lateral X-rays from the<br />

43 subjects of both genders divided in two groups: Experimental Group (Group 1), with 23<br />

patients at a mean initial age of 11.81 years, treated with the fixed functional appliance Twin<br />

Force, for a mean period of 2.49 years and Control Group (Group 2), with 40 lateral X-<br />

rays from 20 patients at a mean initial age of 12.54 years, presenting Class II malocclusion,<br />

observed by a mean period of 2.19 years. It was evaluated the lateral cephalograms before<br />

and after orthodontic treatment in the Group 1 and in the beginning and at the end of the<br />

observation period in the Group 2. Initial and final dentoskeletal cephalometric variables and<br />

changes with treatment were compared between the groups with t-test. The experimental<br />

group presented a greater restriction of growth and anterior displacement of the maxilla<br />

and a greater mandibular retrusion, because there was change in the occlusal plane and<br />

anterior facial height. The Group 1 showed a significant improvement of maxillomandibular<br />

relationship and the mandibular incisors, presented greater buccal inclination.<br />

There were no statistically significant differences in the craniofacial growth pattern between the<br />

groups; the Class II correction occurred almost entirely by dentoalveolar changes then skeletal.<br />

HA010 Free radical concentration analysis via electron paramagnetic<br />

resonance (EPR) for composite resin<br />

Salomão FM*, Peron RAF, Poletto D, Borges HOI, Garbelini CCD,<br />

Vicentin BLS, Di‐Mauro E, Hoeppner MG<br />

Odontologia Restauradora - UNIVERSIDADE ESTADUAL PAULISTA - ARAÇATUBA.<br />

E-mail: salomaofm@me.com<br />

Translucent fiberglass post (FP) capacity to transmit light is a questionable property until<br />

today. Free radical concentration (FRC) formed in a nanoparticulated composite resin (CR),<br />

photopolymerized throughout a FP sectioned in different lengths was investigated using<br />

Electron Paramagnetic Resonance (EPR). 15 CR samples were confectioned, divided in 5<br />

groups (n=3), accordingly to FP length: Positive Control Group (+CG) - Polymerization device,<br />

with 1200mW/cm2 potency, positioned directly over the samples; Negative Control Group<br />

(-CG) - samples were not photopolymerized; Group 1 (G1) - 4mm FRC maintained the<br />

LED 4mm away from the samples, during irradiation for photopolymerization; Group 2<br />

(G2) - 6mm FP maintained the LED 6mm away from the samples, during irradiation for<br />

photopolymerization; Group 3 (G3) - 8mm FP maintained the LED 8mm away from the<br />

samples, during irradiation for photopolymerization. Samples were evaluated immediately<br />

after photopolymerization. EPR Spectra analysis indicated that GC- was the only one not<br />

present in the spectrum visible sign. GC+ presented the greater spectra intensity, that is, greater<br />

FRC. G1 and G2 had similar FRC and that was greater than G3.<br />

FRC declined from the use of FP and with the increasing distance from the LED tip and the<br />

samples.<br />

HA011 Influence of nanoparticle addition on the mechanical properties of<br />

facial silicone subjected to accelerated aging<br />

Nobrega AS*, Andreotti AM, Silva EVF, Sônego MV, Moreno A, Turcio KHL,<br />

Sinhoreti MAC, Goiato MC<br />

Materiais Odontológicos e Prótese - UNIVERSIDADE ESTADUAL PAULISTA - ARAÇATUBA.<br />

E-mail: dha_sn@hotmail.com<br />

The aim of this study was to evaluate the influence of nanoparticles addition in hardness, tear<br />

strength and permanent deformation of Facial Silicone Silastic MDX4-4210. A total of 560<br />

samples of Silastic were made, 280 for tear strength test, 140 for hardness test and 140 for<br />

permanent deformation test. Nanoparticles added in the preparation of silicone samples were<br />

zinc oxide, barium sulfate and titanium dioxide. For each test (except the tear strength test,<br />

in which the samples are duplicated), 40 samples of silicone were made, with the addition of<br />

each type of nanoparticles, totaling 120 samples, 10 samples of silicone with oil paint without<br />

nanoparticles, and 10 samples only of silicone. For each type of nanoparticle added to the<br />

facial silicone, 20 samples were prepared at a concentration of 1%, and 20 at a concentration<br />

of 2%. For each concentration of nanoparticles oil paint was added in half of the samples<br />

(10 samples). Samples were submitted to the tests before and after 1008 hours of accelerated<br />

aging. Data were subjected to statistical analysis using nested ANOVA and Tukey tests. It can<br />

be verified through the results that nanoparticles influenced the hardness, tear strength and<br />

permanent deformation of the assessed groups<br />

It is concluded that all evaluated nanoparticles influenced the hardness, tear strength and<br />

permanent deformation, however the groups with addition of titanium dioxide nanoparticles<br />

exhibited different hardness values of the clinically acceptable range. Accelerated aging<br />

influenced all evaluated properties, regardless the addition of nanoparticles or oil paint (Apoio:<br />

FAPESP - 2012/05270-3)<br />

HA012<br />

Influence of chlorhexidine on bond strength of self-etching adhesives<br />

Andrade CO*, Salvio LA, Goes MF, Resende FO<br />

Ore - UNIVERSIDADE FEDERAL DE JUIZ DE FORA.<br />

E-mail: carol.ufjf@yahoo.com.br<br />

Was investigated the microtensile bond strength of self-etching adhesive systems in dentin<br />

treated with 2% chlorhexidine digluconate (CHX) in self-etching technique (ST) and totaletching<br />

technique (TT). Occlusal surfaces of 20 human molars were removed to expose middle<br />

dentin. Eight groups were created according to treatments: 1A, Clearfil SE (SE) with ST; 1B,<br />

SE with ST and previous application of CHX; 2A, Scotchbond Universal (SBU) with ST; 2B,<br />

SBU with ST and previous application of CHX; 1C, SE with TT; 1D, SE with TT and previous<br />

application of CHX; 2C, SBU with TT; 2D SBU with TT and previous application of CHX.<br />

A block of composite resin was build (5mm) on hybridized surface, and witch sample was<br />

sectioned in 1mm2 sticks and subjected to microtensile test. After fractures, the samples were<br />

evaluated on Scanning Electron Microscope (SEM) and Energy Dispersive X-Ray Analyser<br />

(EDX). ANOVA two-ways and Tukey test (α = 0.05) statistical analysis was performed.<br />

According to results, CHX interfered on ST reducing the bond strength values in groups 1B<br />

(22,86±5,18) and 2B (27,02±5,58) comparing with 1A (39,77±11,56) and 2A (40,84±12,49).<br />

However, the same did not happened in TT, that presented no statistical difference between<br />

groups 1C (25,95±5,43) and 1D (28,09±4,20), 2C (31,53±9,22) and 2D (37,81±11,69).<br />

Analysis by SEM revealed that ST groups presented cohesive adhesive fractures; and TT,<br />

cohesive at the top of the hybrid layer was the most frequent type of fracture. EDX revealed<br />

the presence of CHX crystals for ST, which was not found for TT.<br />

CHX influenced in a negative way on the bond strength of adhesive systems on self-etching<br />

technique.<br />

HA013<br />

Injectability of gray and white Portland cement for dental application<br />

Alandia‐Román CC*, Silame FDJ, Pires‐de‐Souza FCP, Panzeri H<br />

Materiais Dentarios e Prótese - UNIVERSIDADE DE SÃO PAULO - RIBEIRÃO PRETO.<br />

E-mail: ccalandia@usp.br<br />

Portland cement (PC) based Mineral Trioxide Aggregate (MTA) is widely accepted as the<br />

preferred repair material in endodontics. Disposable extrusion devices for MTA are costly<br />

instruments, as an alternative, medical stainless steel needles with similar diameters could be<br />

used. The aim of this study was to evaluate a simple method for placing PC using medical<br />

stainless steel needles and to compare the injectability (I) of white (WPC) and gray PC (GPC).<br />

WPC-GPC containing 20% of Bismuth oxide were mixed with distilled water at powder-toliquid<br />

ratios (PLR) of 3, 3.5 and 4.0g/ml, then placed into a 5ml syringe using needles with<br />

inner diameter of 0.69mm (19G) to deliver the pastes. The percentage of paste extruded was<br />

determined and the compressive strength (CS) and porosity (P) were also evaluated. GPC<br />

was practically fully extruded (I=94.5%) at a PLR of 3g/ml, PLR´s of 3.5 and 4.0g/ml were<br />

still injectable, however, the percentage of extruded cement decreased with increasing PLR´s<br />

(I=84.5% and 60.2%).WPC showed reduced injectability at a PLR of 3g/ml (I=22.9%), PLR´s<br />

of 3.5 and 4.0g/ml were not injectable at all (I=9.8% and 0%). CS and P were inversely<br />

proportional and related to PLR, the increase of water content led to decreased CS and<br />

increased P values for both GPC (CS= 26.9, 39.0and 44.9MPa/ P=22.3, 15.9 and 14.3%) and<br />

WPC (CS= 21.5, 38.0and 40.5MPa/ P=26.3, 18.5 and 13.0%).<br />

It was concluded that only GPC could be made injectable in this study and 19G needles<br />

demonstrated to be a suitable alternative to existing MTA disposable delivery devices. (Apoio:<br />

CAPES)<br />

Braz Oral Res 2014;28(Suppl. 1):22-476 (Proceedings of the 31st SBPqO Annual Meeting) 33

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