Safety evaluation of certain food additives - ipcs inchem

ALIPHATIC BRANCHED-CHAIN SATURATED AND UNSATURATED ALCOHOLS 321
Table 5 (contd)
No. Flavouring agent End-point Test object Concentration Results Reference
Positive f Dearfield et al. (1991)
250–3000 μg/ml, S9; 500–
1000 μg/ml, +S9
Forward mutation L5178Y mouse
lymphoma cells
1834 Methyl 2-methyl-
2-propenoate
Schweikl et al. (1998)
Weakly
positive
10 or 20 mmol/l, S9 (~1000
and 2000 μg/mld )
Gene mutation V79B Chinese hamster
lung fibroblast cells
1834 Methyl 2-methyl-
2-propenoate
Doerr al. (1989)
1000–3000 μg/ml, S9 Weakly
positivek L5178Y mouse
lymphoma cells
Micronuclei
induction
1834 Methyl 2-methyl-
2-propenoate
Schweikl et al. (2001)
Weakly
positivek 10, 20 or 30 mmol/l, S9
(~1000, 2000 and 3000
μg/mld )
V79 Chinese hamster
lung fibroblast cells
Micronuclei
induction
1834 Methyl 2-methyl-
2-propenoate
Positive k Yang et al. (2003)
CHO cells 106 –103 (v/v), S9
(~1–1000 μg/mlb,l )
DNA synthesis
inhibition
1834 Methyl 2-methyl-
2-propenoate
Moore et al. (1988);
Doerr et al. (1989)
1000–3000 μg/ml, S9 Weakly
positivek Mouse lymphoma
L5178Y cells
Chromosomal
aberration
1834 Methyl 2-methyl-
2-propenoate
Anderson et al. (1990)
Positivem ;
weakly positivei CHO cells 16–3000 μg/ml, S9;
160–5000 μg/ml, +S9
Chromosomal
aberration
1834 Methyl 2-methyl-
2-propenoate
Positive k Yang et al. (2003)
CHO cells 106 –103 (v/v), S9
(~1–1000 μg/mlb,l )
Chromosomal
aberration
1834 Methyl 2-methyl-
2-propenoate
Human lymphocytes 0.01 or 0.1 μg/ml, S9 Negative Cannas et al. (1987)
Sister chromatid
exchange
1834 Methyl 2-methyl-
2-propenoate
Positive n Anderson et al. (1990)
CHO cells 5–1250 μg/ml, S9;
50–5000 μg/ml, +S9
Sister chromatid
exchange
1834 Methyl 2-methyl-
2-propenoate