Safety evaluation of certain food additives - ipcs inchem

ALKOXY-SUBSTITUTED ALLYLBENZENES 379
substances in the same species and strain (Miller et al., 1983), the authors
concluded that DNA adduct levels of at least 15 pmol/mg of DNA at 23 days were
required for statistically significant tumorigenesis potential (Phillips et al., 1984). The
authors also noted that, compared with adults, newborn mice showed greater
sensitivity to alkoxy-substituted allylbenzene carcinogenicity.
In a dose-dependent study of formation of methyl eugenol–DNA adducts,
groups of male and female Fischer 344 rats were maintained on diets providing 0,
1, 5 or 50 mg methyl eugenol/kg bw for 28 days. The positive control (DNA harvested
from rats treated with 300 mg methyl eugenol/kg bw) showed clear evidence of DNA
adducts in the liver as measured by 32 P-postlabelling. The dietary vehicle control
and the gavage vehicle control groups showed no evidence of methyl eugenol–DNA
adducts in the liver. In both cases, 32 P-postlabelled adducts were below the limit of
detection of the assay (