Safety evaluation of certain food additives - ipcs inchem

408 ALKOXY-SUBSTITUTED ALLYLBENZENES
Table 4 (contd)
No. Flavouring agent End-point Test object Concentration Results References
1790 Methyl eugenol Reverse mutation E. coli WP2 trp 30, 60, 120 and 300 μg/plate Negatived Sekizawa & Shibamoto
(1982)
1000 μg/disc Negativeg Sekizawa & Shibamoto
(1982)
1790 Methyl eugenol DNA repair B. subtilis H17Rec+ and
M45Rec Positive d,j Schiestl et al. (1989b)
121, 291 and 364 μg/mli (0.68, 1.36 and 2.04 mmol/l)
1790 Methyl eugenol Mitotic recombination Saccharomyces
cerevisiae RS9
National Toxicology
Program (2000)
1790 Methyl eugenol SCE CHO cells 5–250 μg/ml Positivef Negativeg 1790 Methyl eugenol Chromosomal aberration CHO cells 50–500 μg/ml Negatived National Toxicology
Program (2000)
Positive h Howes et al. (1990b)
1790 Methyl eugenol UDS Rat hepatocytes 0.178–8900i (106 –5 × 102 mol/l)
Positive h Chan & Caldwell (1992)
1790 Methyl eugenol UDS Rat hepatocytes 0.178–178 μg/mli (106 –
103 mol/l)
1790 Methyl eugenol UDS Rat hepatocytes 890i (5 × 103 mol/l) Positive Howes et al. (1990a,
1990b)
Positive Burkey et al. (1998)
1790 Methyl eugenol UDS Rat hepatocytes 1.78–178 μg/mli (10–1000
μmol/l)
Positive Burkey et al. (1999)
1790 Methyl eugenol UDS Rat hepatocytes 1.78–178 μg/mli (10–1000
μmol/l)