Safety evaluation of certain food additives - ipcs inchem

546 HYDROXY- AND ALKOXY-SUBSTITUTED BENZYL DERIVATIVES (addendum)
Table 5. Studies of genotoxicity with hydroxy- and alkoxy-substituted benzyl derivatives used as flavouring agents
No. Flavouring agent End-point Test object Concentration Results References
In vitro
Negative a Kajiura (1996)
0.305, 1.22, 4.88, 19.5, 78.1,
313, 1250 and 5000 μg/plate
S. typhimurium TA98,
TA100, TA1535 and
TA1537
Reverse
mutation
1879 Vanillin 3-(l-menthoxy)propane-1,2-diol
acetal
Negative a Kajiura (1996)
E. coli WP2uvrA 0.305, 1.22, 4.88, 19.5, 78.1,
313, 1250 and 5000 μg/plate
Reverse
mutation
1879 Vanillin 3-(l-menthoxy)propane-1,2-diol
acetal
Negative a Van Delft (1998b)
0, 62, 185, 556, 1667 and
5000 μg/plate
S. typhimurium TA98,
TA100, TA1535 and
TA1537
Reverse
mutation
1880 Sodium 4-methoxybenzoyloxyacetate
Negative a Van Delft (1998a)
0, 278, 557, 743, 975, 1300,
1741 and 2321 μg/mlb (0,
1.2, 2.4, 3.2, 4.2, 5.6, 7.5 and
10 mmol/l)
Cultured mouse
lymphoma L5178Y cells
Mouse
lymphoma
forward mutation
1880 Sodium 4-methoxybenzoyloxyacetate
Negative a Van Delft (1998a)
0, 145, 290, 580, 1161 and
2321 μg/mlb (0, 0.625, 1.25,
2.5, 5.0 and 10.0 mmol/l)
Cultured mouse
lymphoma L5178Y cells
Mouse
lymphoma
forward mutation
1880 Sodium 4-methoxybenzoyloxyacetate
Negativec Van Delft &
de Vogel (1998)
Chinese hamster ovary 0, 23, 70, 139, 279, 557,
1114 and 2228 μg/mlb (0,
0.1, 0.3, 0.6, 1.2, 2.4, 4.8 and
9.6 mmol/l)
Chromosomal
aberration
1880 Sodium 4-methoxybenzoyloxyacetate
Negativec Van Delft &
de Vogel (1998)
Chinese hamster ovary 0, 290, 580, 1160 and 2321
μg/mlb (0, 1.25, 2.5, 5 and 10
mmol/l)
Chromosomal
aberration
1880 Sodium 4-methoxybenzoyloxyacetate