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BSA Flow Software Installation and User's Guide - CSI

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4.10.5 Data rate requirements<br />

Below are some recommendations. For a detailed description, please consult<br />

the Reference guide chapter.<br />

Mean <strong>and</strong> RMS velocity<br />

4.10.6 Stop criterion possibilities<br />

If you are looking for average quantities such as mean <strong>and</strong> RMS velocity,<br />

the data rate is not critical, but you should acquire sufficient samples for<br />

reliable statistics. This usually means 1000 - 2000 samples per position, but<br />

depends on the flow characteristics.<br />

To avoid statistical bias, you can use the Controlled Dead Time mode, with<br />

the Dead Time interval set to at least 2 times the integral time scale of the<br />

flow. Alternatively, you can use burst mode <strong>and</strong> select Transit Time<br />

Weighting in the Moments object.<br />

Time series, spectrum <strong>and</strong> correlation functions<br />

For this type of results where the dynamics of fluctuations is investigated,<br />

the data rate should obviously be high. If you expect a dominant frequency<br />

in a spectrum, the data rate should ideally be around 2 π times the highest<br />

frequency you are looking for since the spectrum will fall off at a frequency<br />

of mean data rate/2 π. If you cannot achieve such data rate, the optical<br />

system must be optimised in terms of laser power, receiving optics aperture,<br />

<strong>and</strong> use of forward scatter if possible. Changing from backscatter to forward<br />

scatter typically increases the data rate by at least an order of magnitude.<br />

<strong>BSA</strong> <strong>Flow</strong> software includes several stop criteria to stop the acquisition:<br />

• Sample numbers to stop when the acquired sample numbers is reached<br />

(see group properties in chapter 5).<br />

• Acquisition time to stop when the acquisition time is reached (see<br />

group properties in chapter 5).<br />

• Statistical stop criteria to stop the acquisition on the confidence (see<br />

using confidence limits as stop criteria for data acquisition in chapter 5).<br />

• Cyclic phenomena stop criteria to stop the acquisition either on a<br />

number on particles per phase bin or on the cycle numbers (see<br />

Properties of the Cyclic Phenomena Object in chapter 6.<br />

Note Please note that multiple stop criteria can be made inside the same project:<br />

sample number, elapsed time, etc. The acquisition will stop based on the<br />

criterion met first.<br />

4.11 How to add new data to a project<br />

In <strong>BSA</strong> Application, add the new positions to the position co-ordinates list,<br />

either by typing into the list, or by adding a new region, <strong>and</strong> using the Mesh<br />

Generator to define the new positions.<br />

Click the Run button. Click Run in the Data Overwrite Warning window. In<br />

the Ready to Acquire… window, select Positions, <strong>and</strong> specify the indices of<br />

the new positions:<br />

4-62 <strong>BSA</strong> <strong>Flow</strong> <strong>Software</strong>: The user interface

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