BSA Flow Software Installation and User's Guide - CSI

Select the appropriate aperture plate for the PDA probe to obtain the desired
size range.
Size range as a function of aperture plate, scattering angle and transmitting
optics settings is accessible in the PDA software. Therefore, when deciding
which plate to select, try entering the identifications of the different plates
and see which plate gives the range of measurements best suited for the
expected particle size range.
If the largest range is too small, select a larger focal length front lens for the
receiving probe (this does not affect the velocity range) or reduce beam
separation of the transmitting probe if this is possible (this affects the
velocity range). Correspondingly, if the smallest range is too large, select a
shorter focal length front lens (velocity range neutral) or increase beam
separation (this affects the velocity range).
Unscrew and remove the front lens from the PDA probe.
Unscrew the mounting ring (by using the special tool which is supplied with
the receiving probe) and remove the spring washer.
Remove the aperture plate by pointing the front of the receiving probe
downwards or using a small Allen key. Take care not to touch the lens behind
the aperture plate.
Select the proper aperture plate.
Position and mount the new aperture plate so that the small index pin is in
the notch of the plate.
Insert the spring washer and install the mounting ring again.
Install the front lens again.
7.5 Connecting the PDA Detector Unit
The cable from the PDA probe ends in a junction box with three or four
cables coming out of it. These are connected to the respective fibre inputs on
the back side of the PDA detector unit, and the junction box is fixed to the
back side of the detector unit.
The shutter cable, the calibration fibre cable and the photo-multiplier cables
are connected from the PDA detector unit to the BSA signal processor.
Caution Be careful not to bend the fibres excessively.
Always cover the fibre connectors and the fibre inputs with the protective caps
when the probe is not connected.
Interference filters In the PDA detector unit laser line interference filters in front of the photomultipliers
protect against disturbances from high background illumination
and ensure the separation of colour of the scattered light corresponding to
the colour of the beam pairs.
Fibre optic cable Connect the fibre optic cable supplied with the receiving optics between the
(calibration) threaded receptacle on the back panel of the PDA detector unit (Figure 7-58
and Figure 7-59: Cal. input) and the laser diode output on the back panel of
the BSA signal processor.
7-76 BSA Flow Software: Reference guide