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BSA Flow Software Installation and User's Guide - CSI

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6.9.1 Required hardware<br />

6.9.2 Calibration procedure<br />

The Analog/Digital input Option is required for concentration or temperature<br />

LIF calibration.<br />

This chapter describes how to do a Concentration-LIF calibration. This<br />

procedure is the similar for other calibration like Temperature-LIF or for<br />

other instruments connected to the Analog/Digital Input board requiring a<br />

calibration.<br />

The purpose of the calibration is to calculate the relation between the LIF<br />

signal <strong>and</strong> a known concentration of dye. This relation will be used<br />

afterward as transfer function in the Analog/Digital input properties to<br />

calculate the dye concentration corresponding to the measured LIF signal. In<br />

the following example, the SignalLIF output is connected to the<br />

Analog/Digital Input 4 (AD4)<br />

• Step 1: by adding step by step a known quantity of dye, the<br />

concentration calibration curve, SignalLIF=f (Dye concentration), is<br />

plotted. A Profile Plot is created as a child of the Moments object.<br />

Figure 6-89 Example of Concentration-LIF calibration curve<br />

• Step 2: Transpose the curve to get the following relation<br />

Concentration=f -1 (SignalLIF)<br />

<strong>BSA</strong> <strong>Flow</strong> <strong>Software</strong>:Options <strong>and</strong> Add-ons 6-97

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