BSA Flow Software Installation and User's Guide - CSI

i.e. as a function of particle size (dp) and position, where x’ and y’ are the
particle location in the coordinate system. An example particle trajectory is
shown in Figure 7-76. An effective measurement volume diameter d e , as
illustrated in Figure 7-76 can be defined.
L min
Particle
Trajectory
d e d o
d Vt
Y'
X'
Figure 7-76: Definition of the effective measurement volume diameter (do is
the measurement volume diameter at the 1/e 2 -intensity points).
The burst length is determined as:
for 1D configurations:
L= t⋅
∆ u ( 7-45)
for 2D and 3D configurations
L = ∆t
u + v
2 2 ( 7-46)
where u and v are the measured velocity components of the droplet and ∆t
is the transit or residence time.
The relationship between d e and Vmax will depend on the detection trigger
level of the processor L2, denoted V t in the following equation:
⎪⎧
2
8 ⎛ ⎞ ⎪⎫
− ⎨⎜
⎟
2 ⎬
=
⎪⎩ ⎝ 2 ⎠ ⎪⎭
max e
d e
d o
t V V ( 7-47)
This equation can be solved for d e :
7-114 BSA Flow Software: Reference guide