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BSA Flow Software Installation and User's Guide - CSI

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Step by step procedure<br />

Phase-Doppler Linearity vs Scattering Angle<br />

Input<br />

Enter relative refractive index: Valid range 0.6 - 2.0 1,334<br />

Enter scattering angle: Valid range 2 - 180 30 deg.<br />

Select polarisation:<br />

Output:<br />

Perpendicular Parallel<br />

Confidence: 97,04 % (Confidence of linearity)<br />

Dominant scattering order: 1st order refraction<br />

For the above configuration, the best scattering angle was: 68 deg.<br />

Graph<br />

The graph below indicates the confidence of linearity for a particular particle type:<br />

100<br />

80<br />

60<br />

40<br />

20<br />

0<br />

Confidence vs Scattering Angle<br />

0 20 40 60 80 100 120 140 160 180<br />

All rights reserved. Dantec Dynamics A/S 2002<br />

Data supplied by SLA, Technische Universitat, Darmstadt.<br />

Scattering angle (deg)<br />

Figure 7-62: PDA optical configuration Excell spreadsheet.<br />

1. Enter the relative refractive index<br />

2. Select the laser polarization<br />

3. Select a scattering angle from the graph. The selected scattering angle<br />

should correspond to a confidence higher 90% <strong>and</strong> should be selected<br />

in an area where the confidence is flat. Pics where the confidence<br />

varies too much with scattering angle should be avoided.<br />

4. Enter the selected scattering angle. Read the corresponding<br />

scattering mode.<br />

The user will enter the scattering angle <strong>and</strong> scattering mode in the PDA<br />

receiver properties (chapter 5.8.2 PDA receiver).<br />

Simulations The simulations are based on the Lorenz-Mie theory with a Debye-series<br />

decomposition. The Lorenz-Mie theory describes the interaction between a<br />

plane wave <strong>and</strong> a homogeneous, isotropic <strong>and</strong> spherical particle. Its<br />

decomposition in Debye series allows to interprete the scattered field in term<br />

of scattering mode.<br />

For all particles diameter between 10 <strong>and</strong> 200 µm (by steps of 1 µm), the<br />

percentage of each scattering mode in relation of the total ligth intensity was<br />

calculated. For all the scattering angles <strong>and</strong> each particle, the highest<br />

percentage is selected. Then, the minimum of all the highest percentage is<br />

selected from all the diameters <strong>and</strong> plotted. These calculations were repeated<br />

<strong>BSA</strong> <strong>Flow</strong> <strong>Software</strong>:Reference guide 7-81

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