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BSA Flow Software Installation and User's Guide - CSI

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Phase/diameter<br />

Therefore, to compensate the differences in aperture area, the more intense<br />

laser line can be used for the planar PDA, typically the green line. However<br />

the system will also function well independent of which wavelength is<br />

chosen for the planar PDA, provided a minimum intensity is achieved.<br />

linearity The linearity of the phase/diameter curve is a further factor for choosing<br />

wavelength. Only for a few selected situations, in particular for very low<br />

relative refractive index (m < 1.2), has a discernable difference in linearity<br />

been observed. In these situations the planar PDA showed some<br />

improvement at low particle sizes using the green line.<br />

However the conventional 2D-PDA (ClassicPDA or FiberPDA) uses the<br />

green line for the U-channels. To conform with this, <strong>and</strong> in light of the very<br />

restricted range where lower performance is to be expected, the DualPDA is<br />

delivered such that the green line is used for the U-channels (CPDA) <strong>and</strong> the<br />

blue line for the V-channels (PPDA).<br />

Should the opposite arrangement be desirable it is necessary not only to<br />

rotate the transmitting probe, however also the following modifications are<br />

necessary:<br />

1.) The polarization of the transmitting beams must be rotated by<br />

rotating the fiber connectors on the in-coupling side, i.e. at the<br />

transmitter box (see below also 0 <strong>and</strong> 0 ).<br />

Instead of rotating the transmitting probe <strong>and</strong> the fiber connectors (in<br />

order to maintain the polarization direction) the fiber connectors on the incoupling<br />

side (i.e. at the transmitter box) can be exchanged between the<br />

green <strong>and</strong> the blue channel.<br />

2.) The colour interference filters in the receiving unit, placed just<br />

prior to the photodetectors, must be exchanged between the U- <strong>and</strong> the<br />

V-channels.<br />

Another possibility to achieve a setup having the green beams on the planar<br />

PDA system without the necessity of opening the DualPDA detector unit for<br />

exchange of the interference filters is the following:<br />

1.) Rotate the transmitting probe or exchange the fiber couplers as described<br />

above.<br />

2.) Connect the U1 <strong>and</strong> U2 receiving fibers from the DualPDA probe to the<br />

V1 <strong>and</strong> V2 channels of the DualPDA detector unit. Connect the V1 <strong>and</strong> V2<br />

receiving fibers from the DualPDA probe to the U1 <strong>and</strong> U2 channels of the<br />

detector unit.<br />

7-68 <strong>BSA</strong> <strong>Flow</strong> <strong>Software</strong>: Reference guide

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