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BSA Flow Software Installation and User's Guide - CSI

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Figure 7-47. The geometrical factor β as a function of the scattering angle<br />

ϕ for different relative refractive indices.<br />

Reflection Figure 7-48 shows that, while the geometrical factor for reflection is<br />

independent of the relative refractive index, it is heavily dependent on the<br />

scattering angle. This dependence can be used to supplement or substitute<br />

for changes in ϑ or for changing the aperture plate or the micrometer setting<br />

of the angle adjustment in the ClassicPDA, respectively.<br />

Figure 7-48. The geometrical factor as a function of scattering angle for<br />

light scattered by reflection.<br />

When selecting the scattering angle, however, optimum results are obtained<br />

only in the range of ϕ where reflection is the sole mode of scattering. This<br />

means that ϕ > ϕ c1 <strong>and</strong> ϕ < ϕ r . If you do not know the exact value of n rel ,<br />

you must pick the greatest value of ϕ c1 <strong>and</strong> the smallest value of ϕ r .<br />

7-54 <strong>BSA</strong> <strong>Flow</strong> <strong>Software</strong>: Reference guide

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