BSA Flow Software Installation and User's Guide - CSI

Figure 7-41: The layout of the 112 mm DualPDA Probe (57X90).The two
detectors U1, U2 are in the drawing plane.The two detectors V1, V2 are in
the plane perpendicular to the drawing. The eyepiece is looking at fiber V2.
The FiberPDA and DualPDA Probe collects scattered light from the beam
intersection. This light is guided through four multi-mode optical fibres
each representing a specific viewing angle. The FiberPDA probes uses three
optical fibres and the DualPDA probes uses four optical fibres. All of them
run in a single cable leading to the Detector unit. At the detector unit end the
cable is split into three (FiberPDA) or four (DualPDA), each containing one
fibre.
Components The Fiber and DualPDA Probes carrie the main optical components for the
collection of light:
Front lens.
Aperture plate.
Composite lens.
Alignment eyepiece.
Spatial filter selector (for 57X80 and 57X90 probes only)
The front lens works as a collimator creating a beam of parallel light.
Following the front lens is an aperture plate. This divides the parallel light
beam into three segment corresponding to the photomultupliers U1, U2, U3
for the FiberPDA or into four segments corresponding to the photomultipliers
U1, U2, V1 and V2. Three exchangeable aperture plates are
included for the FiberPDA probe and for the DualPDA probe.
57X40 and 57X50: 60 mm PDA receiving probes
Corresponding to each segment apertures of the aperture plates are focusing
lenses cemented together into a composite lens. Each focuses a segment of
the parallel light beam forming an image of the intersection volume on one
of the slit-shaped spatial filters in front of an optical fibre (Figure 7-38 and
Figure 7-40). The part of the image which falls on the slit itself corresponds
to the probe volume (see 7.9 Moments (one-time statistics). Only the light
from the probe volume is passed by the optical fibre to the photo-multipliers.
57X80 and 57X90: 112 mm PDA receiving probes
The light coming from each aperture (mask) is focused trough a unique
spatial filter (slit) and is colimated to a segmented lens. Each part of this lens
guides the light in a multimode optical fiber. The width of the spatial filter
(25; 50; 100 and 200µm) can be selected by the mean of a slit selector
(Figure 7-39 and Figure 7-41). The part of the image which falls on the slit
itself corresponds to the probe volume (see 7.9 Moments (one-time
statistics). Only the light from the probe volume is passed by the optical
fibre to the photo-multipliers
7-46 BSA Flow Software: Reference guide