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BSA Flow Software Installation and User's Guide - CSI

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The angle of intersection between the two incident beams, ϑ, is determined<br />

by the beam separation, S t , <strong>and</strong> the focal length of the front lens, F t .<br />

Sacttering angle ϕ i is the scattering angle measured from the axis of the transmitting optics<br />

(the bisector of the two incident beams; the Z axis).<br />

Laser beams<br />

ψ i is the azimuth angle giving the rotational position about the Z axis.<br />

polarization The laser beam polarization is defined relatively to the scattering plane. This<br />

plane is defined by the transmitting <strong>and</strong> receiving optics. The polarization is<br />

perpendicular (parallel) when it is perpendicular (parallel) to the scattering<br />

plane. In the Figure 7-18 <strong>and</strong> Figure 7-19, the interference fringes plane in<br />

the (YoZ) plane.<br />

Figure 7-19: Polarization definition.<br />

The factor βi between particle diameter <strong>and</strong> phase shift also depends on the<br />

scattering mode. This is illustrated in Figure 7-20, in which ray tracing has<br />

been used to depict how incident light will scatter from a spherical particle.<br />

Three contributions are included in the representation; reflection from the<br />

outer surface of the particle, refraction through the particle (first order<br />

refraction) <strong>and</strong> refraction with one internal reflection (second order<br />

refraction).<br />

7-24 <strong>BSA</strong> <strong>Flow</strong> <strong>Software</strong>: Reference guide

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