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BSA Flow Software Installation and User's Guide - CSI

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For both orientations of polarization, first order refraction is dominant for<br />

small scattering angles, i.e. its intensity is much greater than that for<br />

reflected light. Thus, a scattering angle around ϕ = 30° (for a water droplet)<br />

can be used with either polarization. Increasing the scattering angle beyond<br />

about 30° results in increasingly different behaviour for the two orientations<br />

of polarization: whereas refraction gets less dominant for perpendicular<br />

polarization, the situation is quite the opposite for parallel polarization.<br />

Due to the Brewster effect on the surface of the droplet, the reflected light is<br />

completely subdued at a scattering angle of ϕ b1 = 73.7° (for a water droplet).<br />

This is therefore a very good angle for measuring the size of water droplets<br />

provided the transmitting optics can be set to parallel polarization. The<br />

useful range for refraction with parallel polarization is hatched horizontally<br />

in Figure 7-45.<br />

Beyond the scattering angle of ϕ c1 = 82.9° (for a water droplet) there is no<br />

longer any first order refraction. Thus, reflection is the only one of the three<br />

modes of scattering present, <strong>and</strong> can be used with perpendicular polarization<br />

in the slant-hatched range of Figure 7-45.<br />

Second order refraction takes over at scattering angles beyond the rainbow<br />

angle, ϕ r = 138.0° (for a water droplet). In general, this mode of scattering<br />

should be used only with great caution. In part of the range, two<br />

components contribute to this mode yielding non-linearities in the size-phase<br />

relationship, <strong>and</strong> in the remaining range there is not so great a difference in<br />

intensity between second order refraction <strong>and</strong> reflection. If the constrictions<br />

are very severe, however, it is possible to make measurements in the range<br />

indicated by vertical hatches in Figure 7-45.<br />

<strong>BSA</strong> <strong>Flow</strong> <strong>Software</strong>:Reference guide 7-51

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