BSA Flow Software Installation and User's Guide - CSI

Alignment
of the eyepiece The focusing of the image onto the slit-shaped spatial filters can be
inspected via an alignment eyepiece. The alignment eyepiece is a lens
magnifying the image of the focusing plane on the fibre end of fiber V1 so
that the DualPDA Probe can be accurately aligned with the beam
intersection volume.
Front lenses A range of front lenses is available. The front lens is a separate unit not
included with the probe. All front lenses for the DualPDA receiving optics
can be used on the 60X60, 60X61, 60X62, 60X63, 60X64, 60X65, 60X66 and
60X67 FiberFlow Probes and vice versa.
Size range The measurable size range is determined by the following parameters:
• Beam intersection angle of the transmitting optics (defined by the
beam separation and the front lens focal length).
• Focal length of the receiving front lens.
• Aperture plate.
• Selected polarization.
• Scattering angle.
Figure 7-42: The image of the beam crossing on the spatial filter.
The 57X80 DualPDA Probe is compatible with the 60X60 and 60X61 Fiber-
Flow probes concerning the use of beam expanders, side-looking section,
probe supports, etc.
Aperture plates The aperture plate defines the receiving apertures. The size range can be
selected by the choice of aperture plate, without changing the focal length of
the front lens or the scattering angle.
Three standard plates are included with the probe (Figure 7-43, Figure 7-44),
A, B and C. The effective spatial (azimuthal) position of each segment, and
thus the particle size sensitivity and range, depends on which plate is used.
The aperture plates are exchangeable.
BSA Flow Software:Reference guide 7-47