Quantitativer Nachweis von Lawsonia intracellularis mittels real-time ...

Material und Methoden
Tabelle 2: Default settings für TaqMan-Sonden in Primer Express ® 3.0
Parameter Value Parameter Value
Primer Tm
Min Primer Tm
Max Primer Tm
Max Difference in Tm of Two
Primers
Primer GC Content
Min Primer %GC Content
Max Primer %GC Content
Max Primer 3' GC's
Primer 3' End Length
Primer 3' GC Clamp Residues
Primer Length
Min Primer Length
Max Primer Length
Optimal Primer Length
Primer Composition
Max Primer G Repeats
Max Num Ambig Residues in
Primer
Primer Secondary Structure
Max Primer Consec Base Pair
Max Primer Total Base Pair
Primer Site Uniqueness
Max % Match in Primer
Max Consec Match in Primer
Max 3' Consec Match in Primer
General
Max Primers / Probes
58
60
2
30
80
2
5
0
9
40
20
3
0
4
8
75
9
7
50
50
Probe Tm
Min Probe Tm
Max Probe Tm
Probe GC Content
Min Probe %GC Content
Max Probe %GC Content
Probe Length
Min Probe Length
Max Probe Length
Probe Composition
Max Probe G Repeats
Max Num Ambig Residues in
Probe
No G at 5' End in Probe
Select Probe with more C's than
G's
Probe Secondary Structure
Max Probe Consec Base Pair
Max Probe Total Base Pair
Amplicon
Min Amplified Region Tm
Max Amplified Region Tm
Min Amplified Region Length
Max Amplified Region Length
68
70
30
80
13
30
3
0
true
true
4
8
0
85
50
150