LIVE POLIO IRUS VACCINES

100 Discussion
in his hands and we were using this as he had
used it.
The viremia that occurred in the animals on
days 1, 2, and 3 was of a titer of 10 2.0 logs, or
perhaps a little bit higher, and about the same
on days 5 and 7.
One of the reasons why we think that this
may be spillover is that we performed a similar
experiment in guinea pigs. We gave guinea
pigs, into the thigh muscles, five milliliters of
Type 1 attenuated virus, which is a rather large
amount for the guinea pigs, and bled them for
days 1, 2, 3, and 4, and found that they had
viremia for that period of time.
DR. BODIAN: I have one more word to say
about "spillover." I might remind Dr. Kirschstein
that we published data some years ago
indicating that with intracardiac inoculation of
large amounts of virus it is difficult, if not impossible,
to recover the virus from the blood
within half an hour. So 1 doubt very much
that the viremia under the circumstances you
mentioned is due to "spillover."
CHAIRMAN BURNET: If there are no further
comments, we will go on to Dr. Sabin's paper,
"Behavior of Cold Mutants of Poliovirus in
Human Beings."