LIVE POLIO IRUS VACCINES

3. A PHYSICAL PROPERTY AS A VIRUS MARKER: DIFFERENCE IN
AVIDITY OF CELLULOSE RESIN FOR VIRULENT (MAHONEY) AND
ATTENUATED (LSc, 2ab) STRAIN OF TYPE 1 POLIOVIRUS
HORACE L. HODES, HELEN D. ZEPP, AND EUGENE A. AINBENDER
The Mount Sinai Hospital, New York, New York
DR. HODES (presenting the paper): The work
which I am about to describe came about as a
result of studies we were making with radioactive
poliovirus. We had grown polioviruses
in the presence of radioactive phosphorus. In
attempting to separate the virus from other materials,
we made use of cellulose resin columns.
The one we finally came to use was the diethylaminoethyl
cellulose resin ion-exchange column
which had been used by Hoyer and his associates.
In working with the viruses it became clear
that the manner in which the viruses were eluted
from this column was quite regular and quite
reproducible.
This gave us the idea that perhaps the pattern
of elution of a virus strain from the DEAE
column was a constant characteristic, and that
if this were so, we might have a physical marker
which could conceivably be correlated with
neurovirulence or lack of it.
We showed very soon that the Type 1 Mahoney
strain came off the column in a very regular
and reproducible way. We obtained from
Dr. Sabin a number of attenuated strains.
The first one we worked with was the LSc,
FILTER ANTIBODY TEST
WITH PURIFIED RADIOACTIVE (P 32 ) POLIO VIRUS
VIRUS IS GROWN
WITH
I VIRUS IS PURIFIED
(=~ ) .. ~~ACTIVE
VIRUS IS ULTRA-
CENTRIFUGED
CHROMATO-
(100,000 g.) GRAPHIC
COLUMN
ROTOR
IMPURE RADIO-
VIRUS
CELLULOSE ION-
EXCHANGE RESIN
(DEAE)
FILTER PAPER
ANTIBODY TEST
RUBBER
STOPPER
fiLTER PAPER
STRIP DIVIDED
BY PENCIL LIN ES
INTO 18 SPACI ES
I Im. EACH
} SERUM
PAPER SPACES ARE MOUNTED
FILTER PAPER CUT INTO 18
SPACES. EACH SPACE
MOUNTED ON A PLANCHET
4
RADIOACTIVITY
is COUNTED
PAPER SPACE
ml
I
PURIFIED
RADIOACTIVE
VIRUS
PURIRED
RADIOACTIVE
VIRUS
INTERVAL
lIME
PRINTER
SCALING
CIRCUIT
FLOW GAS COUNTER
WITH AUTOMATIC
SAMPLE CHANGER
FIG. 1
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