LIVE POLIO IRUS VACCINES

106 Safety-Laboratory Evidence of Attenuation and Safety
TABLE 4. SPINAL NEUROVIRULENCE IN MONKEYS OF ORIGINAL ATTENUATED TYPE 1 POLIOVIRUSES
AND OF 250 C MUTANTS DERIVED FROM THEM
TEMP. PROGENY LOG 10 SPINAL TEST IN MONKEYS
STRAIN
S ROFP .
PROPAGAT.
PASSAGE
NO.
OF
SINGLE
TCD
PER 5 ML 0 METHO
PARALYTOGENIC EFFECT OF. 0.
OF INDICATED DILUTN
ML
PLAQUE
NEEDLE
UNDILUTED
10- L
p 2149 36 8 12 separate 7.3 - 8.1 20 g. 50/60 51/60
pl.ques
25 20 8. 20 g. 0/5
26 g. 0/5
26 la 2 b 8.5 27g. 0/10 0/5
p 2226 36 8 2ab 7.5
2 0 g. 5/5 4/5
4ab 7.5 " 5/5 5/5
25 20 8.2 " 0/5
26 g. 0/5
26 2ab 8.3 27 g. 0/ 10 0/5
L Sc 36 May 2ab 8.0 20 g. 7/30 5/ 20
27 g. 8/10 4/6
30 10 7.7
25 20 7.7
2 7 g. 8/10
2 6 g. 1/5
20 g. 0/5
TABLE 5. SPINAL NEUROVIRULENCE IN MONKEYS OF ORIGINAL ATTENUATED TYPES 2 AND 3
POLIOVIRUSES AND OF 250 C
MUTANTS DERIVED FROM THEM
SPINAL TEST IN MONKEYS
TEMAP. PROGENY LOG 10
OF PASSAGE OF TCD50 PARALYTOGENIC EFFEC 1 OF 0 1
TYPE STRAN TC 5 ML. 0 MPER OD ML. OF INDICATED DILUTION
PROPAGAT. NO. SINGLE PER ML. NEEDLE
UNDILUTED 10- 1
Z P 712 36 15 Zab 7.7 20 g. 1/Z0 0/
27 g. 1 + 4 sl/ 10 1 + 3 .1/ 10
25 Zo 7.7 Z0 g. 0/5
26 g. 0/5
26 Za 2 b 8.7 27 g. 0/ 10 0/5
3 LEON 36 40 2 Z2lb 7.9 Z0 g. 4/ Z0 0/5
27g. 9/10 3/6
25 20 6.7 20 g. I l1/5
26 g. 1/5
GLENN 36 27 7.0 20 g. 3/4
25 20 7.7
2 g. 0/ 5
26 g. 0/5
26 3aib 8. 3 27 g. 0/ 10 0/5