ARUP; ISBN: 978-0-9562121-5-3 - CMBBE 2012 - Cardiff University

order (PB). The particles adhered (green) (Fig. 4c) after 10 min represented a 4% of
particles suspended in media. The local analysis (Fig. 4d) showed that a higher
concentration of particles was found in a pore border in comparison to the number of
particles on the inner cavities (IC).
Fig. 4. Computational implementation (a) Velocity magnitude (b) Shear stress
distributed on scaffold surface (c) Particles adhered (green) and particles suspended
(gray) (d) Distribution of particles adhered on scaffold surface. Magnification of
particles adhered (show adhesion zone)
Fluorescent microscopy was used to observe the live motion of particles in the
transparent chamber (Fig. 5a). In the centre part of the figure, the scaffold is located and
in the live video the particles trajectories and the deposition of particles are shown. An
acceleration of particles can be observed, when they are close to the scaffold surface.
Figure 5b shows the particles distribution after 10 min. The distribution was
homogeneous on the inner scaffold. The density of adhesion was higher on the pore
border in comparison to inner cavities scaffold.
Fig. 5. Experimental approach. (a) Life image of fluorescent particles in motion. (b)
Distribution of particles adhered on scaffold surface.