43. Gartenbauwissenschaftliche Tagung - (DGG) und des

Gemüsebau
Glucosinolates Content Level in Turnip (Brassica rapa L.) Influenced by
Interaction of Nitrogen and Sulphur Supply
S.M. Li 1, 3 , I. Schonhof 2 , A. Krumbein 2 , L. Li 3 , H. Stützel 4 and M. Schreiner 2
1 3
Department of Plant Nutrition, China Agricultural University, Beijing, China, Resource and
Environment College, Northeast Agricultural University, Harbin, China
lishumin113@yahoo.com.cn
2
Institute of Vegetable and Ornamental Crops, Großbeeren/Erfurt e. V., Germany,
4 Institute of Vegetable and Fruit Sciences, University of Hannover, Germany
The glucosinolates (GLS) are a large of nitrogen and sulphur containing compounds which
occur in all economically important of Brassica vegetables. Previous studies have shown that
turnip had high content of health promoting GLS, such as indol GLS and gluconasturtiin. S
supply generally is considered to be the most important factor for affecting the content of
GLS, and S uptake is strongly suppressed by a large amount of N application. But little is
known about the effect of ratio of nitrogen and sulphur on the regulation of the synthesis of
GLS in turnip. Therefore, pot experiments were conducted to investigate different combinations
of N and S applications on GLS content in turnip roots. The experiment consisted of 4
levels of N application (80, 160, 240 and 320 kg N ha -1 donated by N80, N160, N240 and N320)
and 3 levels of S application (10, 20, 60 kg S ha -1 donated by S10, S20 and S60). A modified
HPLC method was used to determine desulfo-glucosinolates content in roots. Significant influence
of N and S application on total and profile of individual GLS was found in pot experiments.
The total GLS content ranged widely from 14.4 to 108.0 mg 100 FW -1 . In each
level of N treatment, total GLS content significantly increased by increasing S supply. With
increasing N supply, total GLS content decreased in low S treatments. The indole GLS and
gluconasturtiin content in turnip roots showed similar trends to the total GLS content. But in
S10 and S20 treatments N supply had no significant influence on indole GLS content. S supply
had no significant influence on N concentration in roots and leaves in each level of N treatment.
In low S supply, S concentration in turnip roots decreased in response to high N supply.
But in high S supply, S concentration in turnip roots increased in response to high N supply.
There was good correlation between total GLS and N: S ratio in roots. Here results indicated
that GLS content was strongly influence by N as well as S supply.
BHGL – Tagungsband 24/2006
197