Software Manual Freedom EVOware Standard 2.3 Freedom ...

A - Appendix A
Advanced Worklist Commands
The following example pipettes, initializes the carousel, gets a plate, executes an
external application and pipettes again:
A;S1;;Microplate, landscape;1;;10
D;D1;;Microplate, landscape;1;;10
W;
B;FACTS("Carousel","Carousel_InitCarousel","","0","");
B;FACTS("Carousel","Carousel_PresentPlate","2,5","1","96_Well_Plate");
B;Execute("c:\notepad.exe",2,"");
A;S1;;Microplate, landscape;2;;10
D;D1;;Microplate, landscape;2;;10
W;
A.15.3 Well Selection
Many of the advanced worklist commands have a parameter called wellSelection.
wellSelection is a string which specifies the wells (tips) which should be used for
the command.
Characters 1 and 2 of the string specify the number of wells in the x-direction in
hexadecimal. Characters 3 and 4 of the the string specify the number of wells in
the y-direction in hexadecimal. For example, 12 x 8 (96 wells) = 0C08.
All following characters are used for the well selection, whereby each character
specifies the well selection for a group of 7 adjacent wells using a specially
adapted bitmap system. Only 7 bits are used per byte instead of 8 to avoid screen
and printer font compatibility problems. Using the 7-bit system, 14 characters are
needed to represent the well selection for 96 wells (plus characters 1 to 4, total of
18 characters) and 55 characters are needed to represent the well selection for
384 wells (total of 59 characters).
In addition, since most ASCII characters below ASCII 32 are non-printable (nonhuman-readable),
decimal 48 (ASCII value for “0”) is added to the value of the
bitmap to make it easier to read, send by eMail etc. The following shows some
examples for character 5 of the well selection string for a 96-well microplate in
landcape orientation.
Character 5 is responsible for the first group of 7 wells, A1 to G1 (wells are
counted from rear to front and then from left to right):
A1 = bin. 0000001 = dec. 1. Add 48, result = 49 (ASCII value for “1”)
B1 = bin. 0000010 = dec. 2. Add 48, result = 50 (ASCII value for “2”)
C1 = bin. 0000100 = dec. 4. Add 48, result = 52 (ASCII value for “4”)
D1 = bin. 0001000 = dec. 8. Add 48, result = 56 (ASCII value for “8”)
E1 = bin. 0010000 = dec. 16. Add 48, result = 64 (ASCII value for “@”)
F1 = bin. 0100000 = dec. 32. Add 48, result = 80 (ASCII value for “P”)
G1 = bin. 1000000 = dec. 64. Add 48, result = 112 (ASCII value for “p”)
A1 + E1 = bin. 0010001 = dec. 17. Add 48, result = 65 (ASCII value for “A”)
etc.
No wells selected = bin. 0000000 = dec. 0. Add 48, result = 48 (ASCII for “0”)
Note: H1 (and A2 to F2) are coded in character 6 of the well selection string, not
character 5.
The above examples result in following well selection strings:
A1 = “0C0810000000000000
B1 = “0C0820000000000000”
A - 120 Freedom EVOware Software Manual, 393172, en, V2.3