Software Manual Freedom EVOware Standard 2.3 Freedom ...

16 - Device Commands
Commands for the Columbus Washer
Aspiration Mode
Choose the required aspiration mode. The available options are normal/
normal, crosswise/crosswise, normal/crosswise and crosswise/normal.
Crosswise aspiration should only be used for 96-well plates with flat bottomed
wells. In this case, aspiration is carried out with the needles at two positions in
each well (front edge and back edge). This minimizes the volume of residual
liquids. For more information, please refer to the corresponding section in the
operating manual of your washer.
Wash mode
Choose whether you want to use plate mode or strip mode. In plate mode,
each process step is performed on all of the strips sequentially before
proceeding to the next process step. The entire microplate is processed for
the defined soaking time. In strip mode, the entire wash program is performed
on a single strip (i.e. one column or row of the microplate, depending on wash
orientation) before proceeding to the next strip. Each strip is processed for the
defined soaking time before proceeding to the next strip. See also
17.5 “Columbus Washer - Washing a Variable Number of Strips in the Plate”,
17-15.
Repetitions
Specify the required number of repetitions of the washing procedure.
Final Asp. Time
Specify the required final aspiration time (range: 1 to 20 seconds). Final
aspiration is used to remove remaining liquid from the wells after the washing
procedure.
Final Asp. Speed
Specify the required final aspiration speed (range: 1 to 20 mm/s). This is the
speed at which the needles are lowered into the wells as the liquid level drops.
16.6.10.2 Wash Tab of the Columbus Washer - WashPlateEx Command
The Wash tab is used to set additional washing parameters.
Fig. 16-23 Columbus WashPlateEx command - Wash tab
Freedom EVOware Software Manual, 393172, en, V2.3 16 - 27