Software Manual Freedom EVOware Standard 2.3 Freedom ...

15 - Script Commands
Serial Dilution Wizard
How many steps should your dilution contain
Specify the required number of steps (range: 2 to 24). Example: if you specify
a dilution of 1:2 per step and a total of 3 steps, the destination plate(s) will all
have three columns of wells with dilution factors of 1:2, 1:4 and 1:8. See also
“Is the source concentration equal to the first step”.
How many dilution series (plates)
Specify how many destination plates you want to make (range: 1 to 16). All of
the destination plates will be identical.
Dilute the first transfer to the destination plate(s)
Click No if you want the first column in the destination plate(s) to contain the
undiluted sample. Example: if you choose No and specify a dilution of 1:2 per
step and a total of 3 steps, the destination plate(s) will all have three columns
of wells with dilution factors of “undiluted”, 1:2 and 1:4.
Is the diluent already distributed
Click Yes if the destination wells in the destination plate(s) already contain
diluent. In this case, you must specify how much diluent is contained in each
well, and the Serial Dilution wizard only needs to aspirate and dispense the
appropriate quantities of the sample liquid. Tip: You can dispense diluent to
the destination wells using the Replicates wizard.
How much diluent is dispensed in each well: Specify how much diluent is
already contained in each destination well.
What final volume do you want to have
Specify the required total volume (sample plus diluent) in each of the
destination wells.
Aspirate sample and diluent together
Click Yes if you want to aspirate the sample directly after aspirating the
diluent. Click No if you want to dispense the diluent into the destination wells
before aspirating and dispensing the sample. Be aware of the risk of
contamination if you choose Yes.
Freedom EVOware Software Manual, 393172, en, V2.3 15 - 71