Software Manual Freedom EVOware Standard 2.3 Freedom ...

16 - Device Commands
Commands for the PW384GP Washer
Vacuum level
Specify the required vacuum level. The vacuum level determines the
aspiration speed.
Plate Type
The PW384GP washer can store parameter sets for up to 15 different type of
labware. The parameter sets are created using the menus on the washer’s
LCD panel. Select the parameter set you want to use for aspirating. For
information on parameter sets please refer to the corresponding section in the
operating manual of your washer.
Head position
Choose the required head position when aspirating.:
Bottom: The aspiration step is performed down to the bottom of the wells to
ensure the minimum volume of residual liquid.
Cell: The aspiration step is only performed down to a predefined height to
leave a small amount of liquid in the wells. This setting is useful for cell
washing. Note: The needle positions “Bottom” and “Cell” are configured in the
washer with the plate parameters.
Aspiration mode
Select the required aspiration mode (standard or crosswise). Crosswise
aspiration should only be used for 96-well plates with flat bottomed wells. In
this case, aspiration is carried out with the needles at two positions in each
well (front edge and back edge). This ensures the minimum volume of
residual liquid. For more information, please refer to the corresponding
section in the operating manual of your washer.
To view or edit the execution parameters again later, right-click on the process
step in the Process Editor and choose Edit Parameters in the context menu.
Objects
The Aspirate command operates on an object of type Sample or a derived type
with default name MP.
Pre-actions and Post-actions
The labware is brought to the PW384GP location with a RoMa MoveObject
command and brought back to its Base location with another RoMa MoveObject
command.
16.18.2 PW384GP Washer - Dispense Command
The Dispense command starts the liquid dispensing procedure of the washer. This
command is mainly intended for special applications, e.g. to fill the wells with
liquid.
Execution parameters
You are prompted to specify the following execution parameters when you add
this command to your script or process:
Freedom EVOware Software Manual, 393172, en, V2.3 16 - 55