Software Manual Freedom EVOware Standard 2.3 Freedom ...

A - Appendix A
Freedom EVO 75 Pipetting Instrument - LiHa Configuration
Example
The following command line starts Freedom EVOware with the user name David
and password Long and runs the maintenance script maintscript.esc:
evoware.exe -m maintscript.esc -u David -w Long
Tip: In Windows, right-click on a shortcut and choose Properties > Shortcut to
assign command line parameters to the shortcut.
A.7 Freedom EVO 75 Pipetting Instrument - LiHa
Configuration
The Freedom EVO 75 pipetting instrument now supports the following LiHa
configurations:
• One tip.
• Two tips.
• 8 Plus 1 Access option.
Please note the following:
Two-tip LiHa
You can select either or both tips in the pipetting commands. The two tips can
be used for pipetting (raised or lowered) independently. The tip spacing is
fixed at 18 mm (every second well of a 96-well microplate).
8 Plus 1 Access option
You can select either tip 1 or all 8 tips in the pipetting commands. Tip 1 can be
used for pipetting (raised or lowered) independently of the other 7 tips. If you
select all 8 tips, they are all used (raised or lowered) together. The tip spacing
is fixed at 9 mm (adjacent wells of a 96-well microplate).
In addition, liquid detection hardware is only provided for tip 1. If you want to
use liquid detection, you must choose the detection mode Detect liquid with
first, tip, move others to same position in the Global settings of the liquid class.
The 8 Plus 1 Access option is currently only intended for disposable tips (not
fixed tips). In addition, you must fit the same DITI type (DITI size) to all 8
positions on the LiHa.
Tip spacing
Tip spreading is not available for the Freedom EVO 75 pipetting instrument (the
tips have a fixed spacing of 9 or 18 mm depending on LiHa type). Accordingly, in
the pipetting commands, you can only change the tip spacing setting for the 2-tip
LiHa configuration. If the spacing between the wells you want to pipette is not
18 mm, pipetting is carried out twice using one tip at a time.
This is not possible with the 8 Plus 1 Access option. If you select all 8 tips, they
are all used (raised or lowered) together. Accordingly, you cannot change the
setting for tip spacing because you cannot use one tip at a time.
Tip spacing is not relevant for the 1-tip LiHa configuration.
Enhanced DITI logistics for the Get DITI command
The following method is used to minimize LiHa movements when getting DITIs (to
speed up the Get DITI commands).
A - 30 Freedom EVOware Software Manual, 393172, en, V2.3