Software Manual Freedom EVOware Standard 2.3 Freedom ...

15 - Script Commands
Te-MO Commands
Fig. 15-89 Te-MO 384 Wash Tip Head command
The parameters of the Te-MO Wash 384 Tip Head command are as follows:
Flush Time
Specify the required flush time (range: 0.1 - 50 seconds). Flushing takes place
with the tips above the Te-MO 384 wash block (tip height = Z-dispense) and
moved slightly to the side to position them in between the cleaner tubes. This
ensures that the cleaner tubes are not contaminated by the liquid which is
expelled during flushing. The specified flush time is preceded by an
automated preflush phase with pressure pulses which takes about 15
seconds. The total time required by this step is thus Flush Time + 15 s.
Quick head flushing
This option speeds up head flushing by a few seconds. Do not use it for the
first wash in the script because a full flush is necessary if you haven’t used the
Te-MO for some time.
Wash Time
Specify the required wash time (range: 0.1 - 50 seconds). Washing takes
place with the tips lowered into cleaning tubes of the Te-MO 384 wash block
(tip height = Z-max). This field is inactive (gray) if you specify Tip washing with
plunger moves.
Tip washing with plunger moves
This option activates an alternative method of washing the tips. It uses
plunger movements to aspirate and dispense wash liquid from the wash block.
The standard method uses the wash block to pump wash liquid through the
pipetting head and the tips but does not move the plunger.
Cycles: Specify how many aspirate/dispense cycles you want (range: 1 to
10).
Volume: Specify the volume of liquid to use for aspirating and dispensing
(range: 1 to 50 µl).
Speed: Specify the required aspirating and dispensing speed (range: 1 to 100
µl/s).
Freedom EVOware Software Manual, 393172, en, V2.3 15 - 131