Software Manual Freedom EVOware Standard 2.3 Freedom ...

15 - Script Commands
MCA384 Commands
Empty Tips step
This step will only be executed if the excess volume or the leading airgap is
still in the tips or the MCA384 plunger has not reached the start position.
During this step, the MCA384 head moves to the Z-dispense position above
the wash block and any remaining liquid is expelled from the tips.
Cleaner step
During each cleaner step, the MCA384 moves the tips into the wash block to
the Z-max position and waits for the specified soak time. The MCA384 then
aspirates and dispenses the specified volume of wash liquid. Specify the
Cycles parameter to repeat aspiration and dispensing several times if
necessary. The WRC washing pumps operate during the entire cleaner step.
The MCA384 head moves up and the washing pumps stop at the end of each
step.
You can specify the wash liquid (1 or 2) you want to use in the wash block. In
addition, the wash block can be flushed for a specified time before starting
(Preflush) and after finishing (Postflush) each step.
Wash position
For each of the steps, click on this table field and choose the required wash
block from the pull down list. The list shows all MCA384 wash blocks which
are currently positioned on the MCA384 in the Worktable Editor (the grid
position and the site of the wash block are shown in brackets). The MCA384
hardware supports up to two MCA384 wash blocks. You can only put MCA384
wash blocks on the carrier type “MCA384 System Carrier”.
Wash liquid
You can connect two different wash liquid containers to the WRC. Specify the
wash liquid you want to use in the wash block.
Flow through [%]
Specify the flow rate of the pressure pump of the WRC (range: 1 - 100%;
100% is the maximum flow rate).
Preflush [s]
Specify how long the wash block should be flushed at the beginning of the
step (range: 0 -1000 s).
Soak time [s]
Specify how long the tips should soak in the wash block before aspirating and
dispensing the wash liquid (range: 0 - 200 s). You must specify at least 1 s for
the last Cleaner step.
Liquid Class
Choose the liquid class of the wash liquid from the pull down list. Click the
Editor button below the table to start the liquid classes editor if you want to
create a new class or modify an existing class (see 10 “Configuring Liquid
Classes”, 10-1).
Volume [µl]
Specify the required volume of wash liquid in the cleaner step. The maximum
allowed volume is the dilutor or DITI capacity (Freedom EVOware does not
automatically handle volumes that exceed the dilutor or DITI capacity).
Cycles
Specifies how many times aspiration and dispensing of the wash liquid takes
place in the cleaner step. If you specify 0, aspiration and dispensing the wash
liquid will not be carried out.
15 - 188 Freedom EVOware Software Manual, 393172, en, V2.3