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Software Manual Freedom EVOware Standard 2.3 Freedom ...

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15 - Script Commands<br />

MCA96 Commands<br />

Compartment in well<br />

Some special labware types (e.g. for protein crystallography) have wells with<br />

several compartments (cavities). <strong>Freedom</strong> <strong>EVOware</strong> can pipette a different<br />

liquid into each of the compartments. Each well typically has one main<br />

compartment and one or more satellite compartments which are directly<br />

adjacent and are often smaller in size. Choose the compartment which you<br />

want to use for pipetting (the main compartment is normally compartment 1).<br />

Well compartments are specified in the labware configuration. This field is<br />

inactive (gray) if the well only has one compartment.<br />

Location<br />

Select the labware which you want to use by clicking on the labware in the<br />

Worktable Editor. The gray (protected) field at the top right of the Location<br />

panel shows the label (name) of the labware that you have selected (it shows<br />

a generic name if you have not assigned a label).<br />

If the MCA96 pipetting head has less tips than the number of wells in the<br />

labware, you must then click in the Location panel to select the set of wells<br />

you want to use. This is not necessary if the pipetting head has the same<br />

number of tips as the number of wells in the labware. Your well selection is<br />

shown in green.<br />

Cursor (x,y)<br />

You can also select wells by entering the x coordinate (from left to right) and<br />

the y coordinate (from top to bottom) of the top left well you want to select. Tip:<br />

The x and y coordinates of each well are shown in a tool tip if you move the<br />

cursor over the well area in the Location panel.<br />

Select: Click this button to select the wells you have specified in the Cursor<br />

(x,y) field. This button has no effect if your well selection does not fit within the<br />

well area of the labware. In addition, it has no effect if the pipetting head has<br />

the same number of tips as the number of wells in the labware.<br />

15.68.11 MCA96 Vector Command<br />

The MCA96 Vector command executes an MCA96 vector, which is a predefined<br />

sequence of MCA96 movements. You can also specify gripper actions at the Safe<br />

and End positions. Gripper actions require the MCA96 gripper option.<br />

MCA96 vectors are very similar to RoMa vectors. See 9.6.1 “Using RoMa<br />

Vectors”, 9-58. The main difference is that the MCA96 gripper fingers cannot be<br />

rotated (they are always parallel to the X axis of the pipetting instrument).<br />

Accordingly, you cannot specify the R axis for MCA96 vectors.<br />

When setting up the hardware, the MCA96 gripper option can be attached to the<br />

MCA96 pipetting head on the left side with the gripper fingers pointing to the right<br />

(under the head) or on the right side with the gripper fingers pointing to the left<br />

(under the head) or to the right (away from the head). On the left side and pointing<br />

to the left is not supported.<br />

The MCA96 Vector command is intended for special MCA96 movements and not<br />

for moving labware from one position to another - you should use the MCA96<br />

Transfer Labware command instead for this purpose. See 15.58.1 “Moving<br />

labware with the RoMa Vector command”, 15-142 for additional information<br />

which is also applicable to the MCA96.<br />

<strong>Freedom</strong> <strong>EVOware</strong> <strong>Software</strong> <strong>Manual</strong>, 393172, en, V<strong>2.3</strong> 15 - 175

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