Software Manual Freedom EVOware Standard 2.3 Freedom ...

10 - Configuring Liquid Classes
Te-MO 96 Liquid Classes
Dispense Position
Specify the Z-position and the Z-offset of the tips when dispensing. A positive
value for the offset lowers the tips.
If you choose a dispense position with tracking, the tips move upwards as the
liquid level rises in the wells during dispensing. This option can be used e.g. to
ensure minimal tip contamination by limiting the tip immersion depth.
You can also choose the X-position when dispensing as follows:
– Center: as specified for the labware which is used
– Left: left of center; the offset is specified with X or Y offset while pipetting
for the labware which is used
– Right: right of center; the offset is specified with X or Y offset while
pipetting for the labware which is used
– Left / Right: left of center for odd columns, right of center for even
columns; the offset is specified with X or Y offset while pipetting for the
labware which is used
– Right / Left: right of center for odd columns, left of center for even
columns; the offset is specified with X or Y offset while pipetting for the
labware which is used
You can also choose the Y-position when dispensing as follows:
– Center: as specified for the labware which is used
– Rear: rear of center; the offset is specified with X or Y offset while pipetting
for the labware which is used
– Front: front of center; the offset is specified with X or Y offset while
pipetting for the labware which is used
– Rear / Front: rear of center for odd columns, front of center for even
columns; the offset is specified with X or Y offset while pipetting for the
labware which is used
– Front / Rear: front of center for odd columns, rear of center for even
columns; the offset is specified with X or Y offset while pipetting for the
labware which is used
By combining an X offset and a Y offset, there are nine possible pipetting
positions (including the center position with no offsets).
Tip Touching
Activate the tip touching function if required and specify the direction of the
movement. The amount of movement is specified with Tip-touching distance
for the labware which is used.
Tip touching is a special movement when dispensing a liquid. When tip
touching is activated, Freedom EVOware dispenses to the destination
container in the normal way, but then moves the tips to the side of the well and
back before retracting them. This helps to remove any droplets which may be
adhering to the tips.
Speed: Specify the tip touching speed.
Delay after touching: Specify the delay between the tip touching the well and
moving back to the middle of the well.
Mix after Dispense
Check this box if you want to mix the liquid after dispensing it. You can specify
the number of mix cycles (range: 1 to 99), the mix volume (max. 2500 µl), the
Z-position and the Z-offset. A positive value for the offset lowers the tips.
Retract Tips to
Specify the Z-position and the Z-offset to which the tips should retract (move
upwards) after dispensing. A positive value for the offset lowers the tips.
10 - 36 Freedom EVOware Software Manual, 393172, en, V2.3