Software Manual Freedom EVOware Standard 2.3 Freedom ...

15 - Script Commands
MCA96 Commands
Volume
Specify the volume that you want to aspirate for the mixing process. The same
volume is pipetted by all of the tips. The maximum allowed volume is the
plunger or DITI capacity (Freedom EVOware does not automatically handle
volumes that exceed the plunger or DITI capacity).
Cycles
Specify how often you want to repeat the mixing process.
Compartment in well
Some special labware types (e.g. for protein crystallography) have wells with
several compartments (cavities). Freedom EVOware can pipette a different
liquid into each of the compartments. Each well typically has one main
compartment and one or more satellite compartments which are directly
adjacent and are often smaller in size. Choose the compartment which you
want to use for pipetting (the main compartment is normally compartment 1).
Well compartments are specified in the labware configuration. This field is
inactive (gray) if the well only has one compartment.
Location
Specify the required labware by clicking on the labware in the Worktable
Editor. You must first put an appropriate labware item in the Worktable Editor
at the required position. The gray (protected) field above the location panel
then shows the label (name) of the labware that you have selected (it shows a
generic name if you have not assigned a label).
If the MCA96 pipetting head has less tips than the number of wells in the
labware, you must click in the Location panel to select the set of wells you
want to use. This is not necessary if the pipetting head has the same number
of tips as the number of wells in the labware. Your selection will be shown in
green.
15.68.4 MCA96 Wash Command
The MCA96 Wash command is used to wash MCA96 tips or MCA96 DITIs using
the specified MCA96 wash block. The MCA96 wash block is intended for 96-tip
MCA96 tip blocks and 96-tip DITi pipetting heads and is connected to the MCA96
Wash & Refill Center (WRC) through tubing. The MCA96 hardware supports up to
two MCA96 wash blocks (each one with its own control unit). You can only put
MCA96 wash blocks on the carrier type “MCA96 Carrier”.
15 - 164 Freedom EVOware Software Manual, 393172, en, V2.3