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Software Manual Freedom EVOware Standard 2.3 Freedom ...

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16 - Device Commands<br />

Commands for the Columbus Washer<br />

Fig. 16-15 Hettich Centrifuge MoveToPos command<br />

Position<br />

Specify the number of the bucket (rotor position) which you want to align with<br />

the transfer position.<br />

16.6 Commands for the Columbus Washer<br />

The Columbus Washer is used for stripwise washing of 96-well microplates<br />

(optional: 384-well microplates). It can be equipped with either a 8, 12 or 16 way<br />

manifold or a 384 way manifold, each of which are provided with one aspirating<br />

and one dispensing needle per well. It can store up to 9 user-defined labware<br />

types that define the dispensing and aspirating positions for round or flat bottomed<br />

plates. The dispensing and aspirating speeds can be selected from 5 pre-defined<br />

speed settings. For dispensing, an additional drip mode setting is available which<br />

allows a wash liquid to be added drop by drop. In the soaking phase, you can also<br />

activate a microplate shaking mechanism. These features allow the device to be<br />

used for all ELISA microplate washing applications including cell washing.<br />

16.6.1 Columbus Washer - WashPlate command<br />

The WashPlate command performs the washing of a microplate.<br />

The washing parameters you specify with this command are used to create a<br />

washing program which is copied to the first program position in the Columbus<br />

washer and then executed. If the first program position in the washer already<br />

contains a user-defined washing program, it is overwritten.<br />

Execution parameters<br />

You are prompted to specify the execution parameters when you add this<br />

command to your script or process. The dialog box has three tabs.<br />

<strong>Freedom</strong> <strong>EVOware</strong> <strong>Software</strong> <strong>Manual</strong>, 393172, en, V<strong>2.3</strong> 16 - 19

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