Software Manual Freedom EVOware Standard 2.3 Freedom ...

10 - Configuring Liquid Classes
LiHa Liquid Classes for Fixed Tips and DiTis
Trailing Airgap after each Dispense
You can automatically aspirate a trailing airgap after each dispense operation
to avoid droplets forming on the tips. The trailing airgap parameters for
dispensing are specified in the Aspirate dialog box.
Use Pinch Valve
This is used to activate the Low Volume option if it is fitted to your pipetting
instrument.
Use Liquid Detection
Check this box if you want to use capacitative liquid level detection (cLLD)
when dispensing. cLLD detects the liquid surface through a change of the
electrical capacitance of the pipetting tip when the tip enters the liquid. Liquid
level detection when dispensing can be used e.g. to ensure that the tips
remain immersed in the liquid during dispensing.
cLLD is carried out with the detection mode, liquid conductivity, detection
speed and double distance settings which you specified on the Global dialog
box for the liquid class.
cLLD must not be used if you are carrying out septum piercing (the septum
interferes with the capacitive detection system).
Dispense Position
Specify the Z-position and the Z-offset of the tips when dispensing. A positive
value for the offset lowers the tips. You can only choose a Z-position relative
to the liquid level if you have activated liquid level detection.
If you choose a dispense position with tracking, the tips will move upwards as
the liquid level rises in the wells during dispensing. This option can be used
e.g. to ensure minimal tip contamination by limiting the tip immersion depth.
You can also choose the X-position when dispensing as follows:
– Center: as specified for the labware which is used
– Left: left of center; the offset is specified with X or Y offset while pipetting
for the labware which is used
– Right: right of center; the offset is specified with X or Y offset while
pipetting for the labware which is used
– Left / Right: left of center for odd columns, right of center for even
columns; the offset is specified with X or Y offset while pipetting for the
labware which is used
– Right / Left: right of center for odd columns, left of center for even
columns; the offset is specified with X or Y offset while pipetting for the
labware which is used
You can also choose the Y-position when dispensing as follows:
– Center: as specified for the labware which is used
– Rear: rear of center; the offset is specified with X or Y offset while pipetting
for the labware which is used
– Front: front of center; the offset is specified with X or Y offset while
pipetting for the labware which is used
– Rear / Front: rear of center for odd columns, front of center for even
columns; the offset is specified with X or Y offset while pipetting for the
labware which is used
– Front / Rear: front of center for odd columns, rear of center for even
columns; the offset is specified with X or Y offset while pipetting for the
labware which is used
By combining an X offset and a Y offset, there are nine possible pipetting
positions (including the center position with no offsets).
Freedom EVOware Software Manual, 393172, en, V2.3 10 - 13