Software Manual Freedom EVOware Standard 2.3 Freedom ...

15 - Script Commands
Worklist Command
The Set DITI Type record can only be used at the very beginning of the worklist or
directly after a Break record. A Break record always resets the DITI type to the
type selected in the Worklist command. Accordingy, if your worklist contains a
Break record, you may need to specify the Set DITI Type record again.
The Comment record (ignored by Freedom EVOware) has the following
structure:
C;Comment
The Reagent Distribution record has the following structure:
R;AspirateParameters;DispenseParameters;Volume;LiquidClass;NoOfDitiRe
uses;NoOfMultiDisp;Direction[;ExcludeDestWell]*
where:
AspirateParameters =
SrcRackLabel;SrcRackID;SrcRackType;SrcPosStart;SrcPosEnd;
and
DispenseParameters =
DestRackLabel;DestRackID;DestRackType;DestPosStart;DestPosEnd;
See 15.24.2.2 “Parameters for Reagent Distribution records”, 15-42 for details
of the parameters.
The Reagent Distribution record specifies the aspiration and dispensing
parameters for all of the tips you have chosen (and dispenses the same volume in
all of the chosen wells in the destination labware).
The Reagent Distribution record has the following advantages when compared to
separate Aspirate and Dispense records:
• Pipetting is automatically optimized during script runtime for the number of tips
which are actually fitted to the LiHa.
• Pipetting is automatically adapted to special LiHa configurations such as the 8
Plus 1 Access option of the Freedom EVO 75 pipetting instrument - it is not
necessary to create a custom, LiHa-specific worklist.
• Worklists are shorter and easier to set up manually using an editor - less risk
of programming errors.
For some examples, see 15.24.6 “Worklist Examples for the Reagent Distribution
Record”, 15-45.
15.24.2.1 Parameters for Aspirate and Dispense records
The parameters for Aspirate and Dispense records are as follows:
Tab. 15-1 Parameters for Aspirate and Dispense records in a worklist file
Parameter Range Explanation
RackLabel Max. 32 characters User-defined label (name) which is assigned to the
labware.
RackID Max. 32 characters Labware barcode.
RackType Max. 32 characters Labware type (configuration name), e.g. “384 Well,
landscape”.
Position 1 .. number of wells Well position in the labware. The position starts with
1 and increases from rear to front and left to right.
TubeID Max. 32 characters Tube barcode.
Volume 0 .. +7158278 Pipetting volume in µl.
Freedom EVOware Software Manual, 393172, en, V2.3 15 - 41