Software Manual Freedom EVOware Standard 2.3 Freedom ...

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A - Appendix A Advanced Worklist Commands The following example pipettes, initializes the carousel, gets a plate, executes an external application and pipettes again: A;S1;;Microplate, landscape;1;;10 D;D1;;Microplate, landscape;1;;10 W; B;FACTS("Carousel","Carousel_InitCarousel","","0",""); B;FACTS("Carousel","Carousel_PresentPlate","2,5","1","96_Well_Plate"); B;Execute("c:\notepad.exe",2,""); A;S1;;Microplate, landscape;2;;10 D;D1;;Microplate, landscape;2;;10 W; A.15.3 Well Selection Many of the advanced worklist commands have a parameter called wellSelection. wellSelection is a string which specifies the wells (tips) which should be used for the command. Characters 1 and 2 of the string specify the number of wells in the x-direction in hexadecimal. Characters 3 and 4 of the the string specify the number of wells in the y-direction in hexadecimal. For example, 12 x 8 (96 wells) = 0C08. All following characters are used for the well selection, whereby each character specifies the well selection for a group of 7 adjacent wells using a specially adapted bitmap system. Only 7 bits are used per byte instead of 8 to avoid screen and printer font compatibility problems. Using the 7-bit system, 14 characters are needed to represent the well selection for 96 wells (plus characters 1 to 4, total of 18 characters) and 55 characters are needed to represent the well selection for 384 wells (total of 59 characters). In addition, since most ASCII characters below ASCII 32 are non-printable (nonhuman-readable), decimal 48 (ASCII value for “0”) is added to the value of the bitmap to make it easier to read, send by eMail etc. The following shows some examples for character 5 of the well selection string for a 96-well microplate in landcape orientation. Character 5 is responsible for the first group of 7 wells, A1 to G1 (wells are counted from rear to front and then from left to right): A1 = bin. 0000001 = dec. 1. Add 48, result = 49 (ASCII value for “1”) B1 = bin. 0000010 = dec. 2. Add 48, result = 50 (ASCII value for “2”) C1 = bin. 0000100 = dec. 4. Add 48, result = 52 (ASCII value for “4”) D1 = bin. 0001000 = dec. 8. Add 48, result = 56 (ASCII value for “8”) E1 = bin. 0010000 = dec. 16. Add 48, result = 64 (ASCII value for “@”) F1 = bin. 0100000 = dec. 32. Add 48, result = 80 (ASCII value for “P”) G1 = bin. 1000000 = dec. 64. Add 48, result = 112 (ASCII value for “p”) A1 + E1 = bin. 0010001 = dec. 17. Add 48, result = 65 (ASCII value for “A”) etc. No wells selected = bin. 0000000 = dec. 0. Add 48, result = 48 (ASCII for “0”) Note: H1 (and A2 to F2) are coded in character 6 of the well selection string, not character 5. The above examples result in following well selection strings: A1 = “0C0810000000000000 B1 = “0C0820000000000000” A - 120 Freedom EVOware Software Manual, 393172, en, V2.3
A - Appendix A Advanced Worklist Commands C1 = “0C0840000000000000” D1 = “0C0880000000000000” E1 = “0C08@0000000000000” F1 = “0C08P0000000000000” G1 = “0C08p0000000000000” A1 + E1 = “0C08A0000000000000” no wells selected = “0C0800000000000000” Note: H1 = “0C0801000000000000” Freedom EVOware can be used to calculate a well selection string for you automatically as follows: Create a simple script which only contains an Aspirate command. Select the wells you want to use and save the script. Script files are saved with the extension .esc in the ..\EVOware\Database\Scripts directory. Open the resulting .esc file with a plain text editor and copy the well string from the parameters of the Aspirate command (see A.15.4.1 “Aspirate command (Worklist: Aspirate)”, A-123). Freedom EVOware Software Manual, 393172, en, V2.3 A - 121
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Software Manual Freedom EVOware ®
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Table of Contents Table of Contents
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Table of Contents 6 Menus 6.1 File
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Table of Contents 8 Configuration T
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Table of Contents 9 Specifying Your
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Table of Contents 11 Getting Starte
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Table of Contents 13.2.40 Commands
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Table of Contents 15.30.2 Serial Di
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Table of Contents 15.68.10 MCA96 Mo
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Table of Contents 16.6.12 Columbus
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Table of Contents 16.27.3 Safire Re
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Table of Contents 17 Special Featur
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Table of Contents 19 Customer Suppo
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Table of Contents B Appendix B - FD
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1 - About This Manual 1 About This
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1 - About This Manual Reference Doc
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1 - About This Manual Context-Sensi
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2 - Safety User Qualification 2 Saf
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2 - Safety Specific Hazards 2.4 Spe
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2 - Safety Safety Features of the P
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3 - Product Description Overview of
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3 - Product Description Intended Us
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3 - Product Description Supported P
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3 - Product Description Computer Re
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4 - Installation Before You Start 4
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4 - Installation Installing Freedom
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4 - Installation Modifying, Removin
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4 - Installation Installing the USB
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4 - Installation Installing the USB
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5 - Starting Freedom EVOware Login
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5 - Starting Freedom EVOware Login
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6 - Menus File Menu 6 Menus This ch
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6 - Menus File Menu Freedom EVOware
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6 - Menus View Menu 6.2.3 Cut / Cop
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6 - Menus Execute Menu Freedom EVOw
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6 - Menus Execute Menu Fig. 6-6 Set
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6 - Menus Administrator Menu 6.4.6.
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6 - Menus Help Menu 6.6 Help Menu T
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6 - Menus Freedom EVOware Toolbar R
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8 - Configuration Tool Device Drive
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11 - Getting Started - Setting Up a
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12 - Running Scripts and Processes
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13 - Overview of Freedom EVOware Co
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15 - Script Commands Aspirate Comma
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15 - Script Commands Mix Command Lo
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15 - Script Commands Activate PMP C
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15 - Script Commands Worklist Impor
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15 - Script Commands Manual Tip Ali
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15 - Script Commands Copy Plate Wiz
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19 - Customer Support 19 Customer S
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A - Appendix A Overview of the Log
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C - Validation of Freedom EVOware A
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D - Glossary Glossary of Terms and
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D - Glossary Important GUI features
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IX - Index IX Index Purpose of This
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IX - Index Notification . . . . . .
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IX - Index for the MCA96 . . . . .
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IX - Index LICOS (overview) . . . .
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IX - Index R Rack (overview) . . .
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IX - Index Te-Shake (overview) . .
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