Software Manual Freedom EVOware Standard 2.3 Freedom ...

16 - Device Commands
Commands for the Magnetic Bead Separator
Fig. 16-72 Magnetic Bead Separation Execution command
To add a command to the separation script, click one of the Commands buttons
after specifying the command parameters to the right of the button. To change the
order of execution of the commands, select a command from the list and click the
Up or Down buttons. To delete a command from the list, select the command and
click Delete.
The following commands are available:
Magnetic Mix
This command mixes the magnetic beads with the sample liquid by applying
the magnet block to alternate sides of the carrier the specified number of
times (Cycles) with the specified waiting time at each of the two positions. In
addition to moving from left to right and back, the magnet block also moves up
and down to follow an approximately rectangular path.
Z-Position
Specify the required Z-position (highest position) of the magnet block for the
Magnetic Mix command (range: 1 to 31 movement steps).
z = 31 is the highest possible position and results in the maximum up and
down movement of the magnet block during the Magnetic Mix command. z = 1
is the lowest possible position and corresponds to the Dispense position. In
this case, the Magnetic Mix command will only move the magnet block from
left to right and back and not up and down.
Specify a smaller value for Z-position if you only have a small volume of liquid
in the labware. This ensures that the upwards movement of the magnet block
will not drag the magnetic beads above the liquid surface. This makes it easier
to aspirate the supernatant or wash liquid with the LiHa in a following step.
This field is only shown if you have checked Support Z-positioning in the
configuration of the Te-MagS driver. This feature requires special Te-MagS
hardware.
Incubation
This command incubates the labware on the heater for the specified time.
Freedom EVOware Software Manual, 393172, en, V2.3 16 - 103