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Software Manual Freedom EVOware Standard 2.3 Freedom ...

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16 - Device Commands<br />

Commands for the Magnetic Bead Separator<br />

Measurement parameters<br />

For each measurement step you want to perform, check the corresponding<br />

checkbox and click Edit. The RdrOLE reader interface software then prompts<br />

you to specify the measurement parameters. Refer to the RdrOLE online help<br />

and manual for more information.<br />

Note: RdrOLE must be configured to connect to the chosen reader. In <strong>Freedom</strong><br />

<strong>EVOware</strong>’s offline mode, you must start RdrOLE and choose the reader manually.<br />

Write raw data to file<br />

Enter the path and name of the raw data file or click Browse and choose an<br />

existing file. A default file name will be suggested automatically. See<br />

16.42 “Specifying Output Filenames for Reader Data”, 16-128 for a<br />

description of the placeholders which can be used to generate reader data file<br />

names.<br />

To view or edit the execution parameters again later, right-click on the process<br />

step in the Process Editor and choose Edit Parameters in the context menu.<br />

Objects<br />

The Multilabel command operates on an object of type Sample or a derived type<br />

with default name MP.<br />

Pre-actions and Post-actions<br />

The labware is brought to the Sunrise location with a RoMa MoveObject<br />

command and the door of the reader is opened with the Open command. After the<br />

measurement, the labware is brought back to its Base location with another RoMa<br />

MoveObject command and the door is closed with the Close command.<br />

16.32 Commands for the Magnetic Bead Separator<br />

The Magnetic Bead Separator option (also called Te-MagS) is a magnetic bead<br />

separator with an optional heating function. It uses a commercially-available liquid<br />

containing microscopic magnetic beads to rapidly isolate bio-molecules (e.g.<br />

DNA, RNA, proteins etc.) or whole cells from various crude mixtures by means of<br />

magnetic forces. The magnetic beads are pulled through the liquid in the tubes<br />

using magnets and collect the required substance as they move.<br />

16.32.1 Magnetic Bead Separator - MoveToPosition Command<br />

The MoveToPosition command moves the carrier to one of four positions. You<br />

must specify the required position.<br />

Execution parameters<br />

You are prompted to specify the following execution parameters when you add<br />

this command to your script or process:<br />

16 - 100 <strong>Freedom</strong> <strong>EVOware</strong> <strong>Software</strong> <strong>Manual</strong>, 393172, en, V<strong>2.3</strong>

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