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Software Manual Freedom EVOware Standard 2.3 Freedom ...

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10 - Configuring Liquid Classes<br />

MCA384 Liquid Classes<br />

Use Liquid Detection<br />

The MCA384 does not support liquid level detection for dispensing directly. If<br />

you want to use a liquid class for the MCA384 which specifies liquid level<br />

detection, the liquid level must be emulated using a special variable (see<br />

14.1.4.14 “RACK_VOLUME_x”, 14-7). Check this checkbox if you want to<br />

activate the emulated liquid level detection. Liquid level detection can be used<br />

e.g. to ensure minimal tip contamination by limiting the tip immersion depth.<br />

For <strong>Freedom</strong> <strong>EVOware</strong> Plus, see 14.1.4 “Pre-Defined Variables”, 14-4 for<br />

information on accessing the current value of this pre-defined variable in<br />

another script.<br />

Dispense Position<br />

Specify the Z-position and the Z-offset of the tips when dispensing. A positive<br />

value for the offset lowers the tips.<br />

If you choose a dispense position with tracking, the tips move upwards as the<br />

liquid level rises in the wells during dispensing. This option can be used e.g. to<br />

ensure minimal tip contamination by limiting the tip immersion depth.<br />

You can also choose the X-position when dispensing as follows:<br />

– Center: as specified for the labware which is used<br />

– Left: left of center; the offset is specified with X or Y offset while pipetting<br />

for the labware which is used<br />

– Right: right of center; the offset is specified with X or Y offset while<br />

pipetting for the labware which is used<br />

– Left / Right: left of center for odd columns, right of center for even<br />

columns; the offset is specified with X or Y offset while pipetting for the<br />

labware which is used<br />

– Right / Left: right of center for odd columns, left of center for even<br />

columns; the offset is specified with X or Y offset while pipetting for the<br />

labware which is used<br />

You can also choose the Y-position when dispensing as follows:<br />

– Center: as specified for the labware which is used<br />

– Rear: rear of center; the offset is specified with X or Y offset while pipetting<br />

for the labware which is used<br />

– Front: front of center; the offset is specified with X or Y offset while<br />

pipetting for the labware which is used<br />

– Rear / Front: rear of center for odd columns, front of center for even<br />

columns; the offset is specified with X or Y offset while pipetting for the<br />

labware which is used<br />

– Front / Rear: front of center for odd columns, rear of center for even<br />

columns; the offset is specified with X or Y offset while pipetting for the<br />

labware which is used<br />

By combining an X offset and a Y offset, there are nine possible pipetting<br />

positions (including the center position with no offsets).<br />

10 - 28 <strong>Freedom</strong> <strong>EVOware</strong> <strong>Software</strong> <strong>Manual</strong>, 393172, en, V<strong>2.3</strong>

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