Software Manual Freedom EVOware Standard 2.3 Freedom ...

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10 - Configuring Liquid Classes Te-MO 96 Liquid Classes Dispense Position Specify the Z-position and the Z-offset of the tips when dispensing. A positive value for the offset lowers the tips. If you choose a dispense position with tracking, the tips move upwards as the liquid level rises in the wells during dispensing. This option can be used e.g. to ensure minimal tip contamination by limiting the tip immersion depth. You can also choose the X-position when dispensing as follows: – Center: as specified for the labware which is used – Left: left of center; the offset is specified with X or Y offset while pipetting for the labware which is used – Right: right of center; the offset is specified with X or Y offset while pipetting for the labware which is used – Left / Right: left of center for odd columns, right of center for even columns; the offset is specified with X or Y offset while pipetting for the labware which is used – Right / Left: right of center for odd columns, left of center for even columns; the offset is specified with X or Y offset while pipetting for the labware which is used You can also choose the Y-position when dispensing as follows: – Center: as specified for the labware which is used – Rear: rear of center; the offset is specified with X or Y offset while pipetting for the labware which is used – Front: front of center; the offset is specified with X or Y offset while pipetting for the labware which is used – Rear / Front: rear of center for odd columns, front of center for even columns; the offset is specified with X or Y offset while pipetting for the labware which is used – Front / Rear: front of center for odd columns, rear of center for even columns; the offset is specified with X or Y offset while pipetting for the labware which is used By combining an X offset and a Y offset, there are nine possible pipetting positions (including the center position with no offsets). Tip Touching Activate the tip touching function if required and specify the direction of the movement. The amount of movement is specified with Tip-touching distance for the labware which is used. Tip touching is a special movement when dispensing a liquid. When tip touching is activated, Freedom EVOware dispenses to the destination container in the normal way, but then moves the tips to the side of the well and back before retracting them. This helps to remove any droplets which may be adhering to the tips. Speed: Specify the tip touching speed. Delay after touching: Specify the delay between the tip touching the well and moving back to the middle of the well. Mix after Dispense Check this box if you want to mix the liquid after dispensing it. You can specify the number of mix cycles (range: 1 to 99), the mix volume (max. 2500 µl), the Z-position and the Z-offset. A positive value for the offset lowers the tips. Retract Tips to Specify the Z-position and the Z-offset to which the tips should retract (move upwards) after dispensing. A positive value for the offset lowers the tips. 10 - 36 Freedom EVOware Software Manual, 393172, en, V2.3
10 - Configuring Liquid Classes Te-MO 96 Liquid Classes Retract Speed Specify the required retract speed. 10.5.3 Te-MO 96 Calibration Parameters Freedom EVOware uses these parameters for the 96-tip Te-MO tip block or DITI pipetting head to ensure that the specified net volumes are dispensed accurately. The two curves in the dialog box show graphically the current settings for single pipetting mode and multi pipetting mode. Fig. 10-17 Te-MO 96 Calibrate parameters for the tip block and DITIs The parameters of this dialog box are as follows (some of the parameters must be specified separately for single pipetting mode and multi pipetting mode): Offset / Factor Specify an offset and/or calibration factor to be applied for single pipetting mode and multi pipetting mode. Freedom EVOware uses the following expression to calculate the Te-MO plunger movement from the net volume specified in the pipetting script: Calibrated Te-MO plunger movement = (net volume * factor) + offset Freedom EVOware Software Manual, 393172, en, V2.3 10 - 37
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Software Manual Freedom EVOware ®
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Table of Contents Table of Contents
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Table of Contents 6 Menus 6.1 File
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Table of Contents 8 Configuration T
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Table of Contents 9 Specifying Your
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Table of Contents 11 Getting Starte
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Table of Contents 13.2.40 Commands
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Table of Contents 15.30.2 Serial Di
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Table of Contents 15.68.10 MCA96 Mo
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Table of Contents 16.6.12 Columbus
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Table of Contents 16.27.3 Safire Re
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Table of Contents 17 Special Featur
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Table of Contents 19 Customer Suppo
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Table of Contents B Appendix B - FD
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1 - About This Manual 1 About This
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1 - About This Manual Reference Doc
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1 - About This Manual Context-Sensi
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2 - Safety User Qualification 2 Saf
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2 - Safety Specific Hazards 2.4 Spe
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2 - Safety Safety Features of the P
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2 - Safety Specific Safety Rules op
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3 - Product Description Overview of
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3 - Product Description Intended Us
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3 - Product Description Supported P
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3 - Product Description Computer Re
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4 - Installation Before You Start 4
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4 - Installation Installing Freedom
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4 - Installation Modifying, Removin
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4 - Installation Modifying, Removin
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4 - Installation Installing the USB
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4 - Installation Installing the USB
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5 - Starting Freedom EVOware Login
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5 - Starting Freedom EVOware Login
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5 - Starting Freedom EVOware Startu
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5 - Starting Freedom EVOware Overvi
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6 - Menus File Menu 6 Menus This ch
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6 - Menus File Menu 6.1.2 Open Free
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6 - Menus File Menu Views Right cli
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6 - Menus File Menu Freedom EVOware
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6 - Menus View Menu 6.2.3 Cut / Cop
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6 - Menus Execute Menu Freedom EVOw
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6 - Menus Execute Menu Fig. 6-6 Set
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6 - Menus Administrator Menu 6.4.6.
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6 - Menus Help Menu 6.6 Help Menu T
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6 - Menus Freedom EVOware Toolbar R
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6 - Menus Freedom EVOware Toolbar D
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7 - Tecan User Management System 7
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8 - Configuration Tool Toolbar Butt
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8 - Configuration Tool Device Drive
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12 - Running Scripts and Processes
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12 - Running Scripts and Processes
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13 - Overview of Freedom EVOware Co
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14 - Advanced Programming Features
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15 - Script Commands Aspirate Comma
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15 - Script Commands Dispense Comma
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15 - Script Commands Mix Command Lo
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15 - Script Commands Mix Command ad
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15 - Script Commands Wash Tips Comm
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15 - Script Commands Periodic Wash
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15 - Script Commands Get DITIs Comm
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15 - Script Commands Set DITI Posit
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15 - Script Commands Pick Up DITIs
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15 - Script Commands Pickup ZipTip
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15 - Script Commands Detect Liquid
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15 - Script Commands Te-Fill Aspira
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15 - Script Commands Te-Fill Dispen
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15 - Script Commands Te-Fill Prime
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15 - Script Commands Activate PMP C
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15 - Script Commands Tip Alignment
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15 - Script Commands Move LiHa Comm
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15 - Script Commands Waste Command
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15 - Script Commands Worklist Comma
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15 - Script Commands Worklist Impor
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15 - Script Commands Manual Tip Ali
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15 - Script Commands MCA Merge Plat
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15 - Script Commands Start Timer Co
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15 - Script Commands Set Variable C
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15 - Script Commands Export Variabl
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15 - Script Commands Execute VB Scr
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15 - Script Commands Start Script C
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15 - Script Commands Resume Command
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15 - Script Commands Te-MO Commands
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15 - Script Commands RoMa Vector Co
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15 - Script Commands MCA384 Command
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16 - Device Commands Specifying Out
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17 - Special Features, Example Scri
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18 - Error Handling Error Dialogs 1
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19 - Customer Support 19 Customer S
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19 - Customer Support USA (Componen
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A - Appendix A Overview of the Log
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A - Appendix A Structure of the Bar
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A - Appendix A Parameters in the Ev
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A - Appendix A Freedom EVOware Dire
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A - Appendix A Command Line Paramet
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A - Appendix A Frequently Asked Que
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A - Appendix A System Tools for Fre
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A - Appendix A LogViewer Tool Fig.
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A - Appendix A LogViewer Tool Docki
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A - Appendix A LogViewer Tool A.10.
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A - Appendix A Tecan Driver Install
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A - Appendix A Common Notification
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A - Appendix A ASTM Interface Modul
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A - Appendix A Configuring and Usin
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A - Appendix A Advanced Worklist Co
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B - Appendix B - FDA 21 CFR Part 11
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C - Validation of Freedom EVOware A
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D - Glossary Glossary of Terms and
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D - Glossary Important GUI features
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D - Glossary Important GUI features
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IX - Index IX Index Purpose of This
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IX - Index D-18 Centrifuge (overvie
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IX - Index Notification . . . . . .
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IX - Index for the MCA96 . . . . .
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IX - Index LICOS (overview) . . . .
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IX - Index R Rack (overview) . . .
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IX - Index Te-Shake (overview) . .
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