Software Manual Freedom EVOware Standard 2.3 Freedom ...

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16 - Device Commands Commands for the PW384GP Washer Fig. 16-43 PW384GP Washer Dispense command Liquid channel Choose the channel (liquid container) which contains the required washing liquid. Choose Rinse if you want to use the rinsing liquid (normally distilled water). Volume Specify the volume (in µl) to be dispensed. The volume depends on the labware type. Speed Specify the required dispense speed (range: 1 to 5). Choose “1” for drip mode. Head position Choose the required head position when dispensing: Overflow: The aspiration needles are slightly below the shoulder (top edge) of the wells. Cell: The aspiration needles are slightly above the bottom of the wells. Note: The needle positions “Overflow” and “Cell” are configured in the washer with the plate parameters. Plate Type The PW384GP washer can store parameter sets for up to 15 different type of labware. The parameter sets are created using the menus on the washer’s LCD panel. Select the parameter set you want to use for washing. For information on parameter sets please refer to the corresponding section in the operating manual of your washer. To view or edit the execution parameters again later, right-click on the process step in the Process Editor and choose Edit Parameters in the context menu. Objects The Dispense command operates on an object of type Sample or a derived type with default name MP. Pre-actions and Post-actions The labware is brought to the PW384GP location with a RoMa MoveObject command and brought back to its Base location with another RoMa MoveObject command. 16 - 56 Freedom EVOware Software Manual, 393172, en, V2.3
16 - Device Commands Commands for the PW384GP Washer 16.18.3 PW384GP Washer - Prime Command Before the washer is used, all dispensing channels needed for the wash step must be primed to fill the liquid system with the required liquid. The Prime command starts the priming procedure. Priming can take several minutes so you should start priming with this command at an early stage in your script or process to make sure that the washer is ready when you want to use it. Execution parameters You are prompted to specify the following execution parameters when you add this command to your script or process: Fig. 16-44 PW384GP Washer Prime command Prime method Choose the required prime method: Full: In this mode, first the dispense needles are flushed. Then the waste tub is filled with liquid. After that the head is lowered into the liquid and the aspirate needles are flushed. Short: In this mode, only the dispense needles are flushed. Short priming is normally carried out using the same liquid which will be used in the wash or dispense command which follows the prime command. Time Specify how long to prime in seconds. Prime channel Choose the channel (liquid container) which contains the required priming liquid. Choose Rinse if you want to use the rinsing liquid (normally distilled water). Waste channel Choose the channel (liquid container) you want to use for the waste liquids. This command has no objects, pre-actions or post-actions. 16.18.4 PW384GP Washer - Rinse Command The Rinse command starts the rinsing procedure of the washer to flush the liquid system. Freedom EVOware Software Manual, 393172, en, V2.3 16 - 57
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Software Manual Freedom EVOware ®
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Table of Contents Table of Contents
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Table of Contents 6 Menus 6.1 File
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Table of Contents 8 Configuration T
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Table of Contents 9 Specifying Your
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Table of Contents 11 Getting Starte
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Table of Contents 13.2.40 Commands
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Table of Contents 15.30.2 Serial Di
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Table of Contents 15.68.10 MCA96 Mo
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Table of Contents 16.6.12 Columbus
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Table of Contents 16.27.3 Safire Re
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Table of Contents 17 Special Featur
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Table of Contents 19 Customer Suppo
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Table of Contents B Appendix B - FD
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1 - About This Manual 1 About This
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1 - About This Manual Reference Doc
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1 - About This Manual Context-Sensi
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2 - Safety User Qualification 2 Saf
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2 - Safety Specific Hazards 2.4 Spe
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3 - Product Description Overview of
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4 - Installation Before You Start 4
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4 - Installation Installing Freedom
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4 - Installation Installing the USB
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5 - Starting Freedom EVOware Login
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6 - Menus File Menu 6 Menus This ch
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6 - Menus File Menu Freedom EVOware
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6 - Menus Execute Menu Freedom EVOw
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6 - Menus Administrator Menu 6.4.6.
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6 - Menus Help Menu 6.6 Help Menu T
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11 - Getting Started - Setting Up a
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13 - Overview of Freedom EVOware Co
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15 - Script Commands Aspirate Comma
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15 - Script Commands Dispense Comma
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15 - Script Commands Mix Command Lo
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15 - Script Commands Wash Tips Comm
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15 - Script Commands Periodic Wash
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15 - Script Commands Set DITI Posit
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15 - Script Commands Pick Up DITIs
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15 - Script Commands Pickup ZipTip
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15 - Script Commands Detect Liquid
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15 - Script Commands Te-Fill Aspira
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15 - Script Commands Te-Fill Prime
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15 - Script Commands Activate PMP C
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15 - Script Commands Tip Alignment
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15 - Script Commands Waste Command
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15 - Script Commands Worklist Impor
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15 - Script Commands Manual Tip Ali
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15 - Script Commands Copy Plate Wiz
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15 - Script Commands MCA96 Commands
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18 - Error Handling Error Dialogs 1
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19 - Customer Support 19 Customer S
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A - Appendix A Overview of the Log
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A - Appendix A Structure of the Bar
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A - Appendix A Command Line Paramet
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C - Validation of Freedom EVOware A
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D - Glossary Glossary of Terms and
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D - Glossary Important GUI features
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IX - Index IX Index Purpose of This
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IX - Index D-18 Centrifuge (overvie
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IX - Index Notification . . . . . .
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IX - Index for the MCA96 . . . . .
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IX - Index LICOS (overview) . . . .
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IX - Index R Rack (overview) . . .
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IX - Index Te-Shake (overview) . .
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