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Software Manual Freedom EVOware Standard 2.3 Freedom ...

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16 - Device Commands<br />

Commands for the Hettich Centrifuge<br />

16.5.2 Hettich Centrifuge - Open Command<br />

The Open command opens the door of the centrifuge.<br />

This command has no execution parameters or objects, pre-actions or postactions.<br />

16.5.3 Hettich Centrifuge - Close Command<br />

The Close command closes the door of the centrifuge.<br />

This command has no execution parameters or objects, pre-actions or postactions.<br />

16.5.4 Hettich Centrifuge - ExecuteN Command<br />

The ExecuteN command carries out centrifuging with N rotor positions of the<br />

device occupied by labware (N = 1, 2, 3 or 4). After the microplates have been<br />

placed into the device and the door closed, the centrifuge will start rotating with<br />

the specified parameters.<br />

To avoid mechanical imbalance, microplates must be put into the centrifuge in<br />

pairs. For example, if you only want to centrifuge one microplate (Execute1<br />

command), you must put a second microplate in the opposite rotor position of the<br />

centrifuge. You can do this by specifying appropriate RoMa MoveObject<br />

commands in the custom pre-actions and post-actions. However, if you always<br />

want to centrifuge just one microplate, it is easier and more efficient to put a<br />

second microplate into the centrifuge by hand.<br />

The Execute1 command puts the microplate in rotor position 1 – to avoid<br />

imbalance you must put another microplate in rotor position 3. The Execute2<br />

command puts microplates in rotor positions 1 and 3 (a balancing microplate is<br />

not necessary). The Execute3 command puts microplates in rotor positions 1, 2<br />

and 3 – to avoid imbalance you must put another microplate in rotor position 4.<br />

The Execute4 command puts microplates in rotor positions 1, 2, 3 and 4 (a<br />

balancing microplate is not necessary).<br />

Execution parameters<br />

You are prompted to specify the following execution parameters when you add<br />

this command to your script or process:<br />

Fig. 16-14 Hettich Centrifuge ExecuteN command<br />

<strong>Freedom</strong> <strong>EVOware</strong> <strong>Software</strong> <strong>Manual</strong>, 393172, en, V<strong>2.3</strong> 16 - 17

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