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Software Manual Freedom EVOware Standard 2.3 Freedom ...

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16 - Device Commands<br />

Commands for the GENios Reader<br />

16.8.6 GENios Reader - Measure Command<br />

The Measure command performs a measurement procedure using external<br />

software. For a detailed description of the parameters of this command please<br />

consult the documentation for RdrOLE and XFluor4.<br />

Execution parameters<br />

The RdrOLE reader interface software prompts you to specify the measurement<br />

parameters when you add this command to your script or process. Refer to the<br />

RdrOLE online help and manual for more information.<br />

Note: RdrOLE must be configured to connect to the chosen reader. In <strong>Freedom</strong><br />

<strong>EVOware</strong>’s offline mode, you must start RdrOLE and choose the reader manually.<br />

After making your entries in the Measurement Parameters dialog box and clicking<br />

Ok, the Output dialog box is shown where you must specify the name of the<br />

output file for the measurements. Alternatively, click Browse and choose an<br />

existing file. A default file name will be suggested automatically:<br />

Fig. 16-28 Specifying the output file of the GENios Reader Measure command<br />

See 16.42 “Specifying Output Filenames for Reader Data”, 16-128 for a<br />

description of the placeholders which can be used to generate reader data file<br />

names.<br />

To view or edit the execution parameters again later, right-click on the process<br />

step in the Process Editor and choose Edit Parameters in the context menu.<br />

Objects<br />

The Measure command operates on an object of type Sample or a derived type<br />

with default name MP.<br />

Pre-actions and post-actions<br />

The labware is brought to the GENios location with a RoMa MoveObject<br />

command and the door of the reader is opened with the Open command. After the<br />

measurement, the labware is brought back to its Base location with another RoMa<br />

MoveObject command and the door is closed with the Close command.<br />

16.8.7 GENios Reader - Multilabel Command<br />

The Multilabel command performs up to four consecutive measurement steps on<br />

the same labware using external software. The labware does not have to leave<br />

the reader in between measurements. Multi labeling allows different components<br />

(labelled with different fluorophores) to be detected in one operation.<br />

16 - 34 <strong>Freedom</strong> <strong>EVOware</strong> <strong>Software</strong> <strong>Manual</strong>, 393172, en, V<strong>2.3</strong>

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